Species diversity of pin nematodes (Paratylenchus spp.) from potato growing regions of southern Alberta, Canada

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dc.contributor.authorMunawar, Maria
dc.contributor.authorYevtushenko, Dmytro
dc.contributor.authorPalomares-Rius, Juan E.
dc.contributor.authorCastillo, Pablo
dc.date.accessioned2024-09-13T20:23:23Z
dc.date.available2024-09-13T20:23:23Z
dc.date.issued2021
dc.descriptionOpen access article. Creative Commons Attribution 4.0 International license (CC BY 4.0) applies
dc.description.abstractPin nematodes (Paratylenchus spp.) are polyphagous parasitic species with a wide host range and geographical distribution; their diversity is unknown in the potato growing region of Alberta, Canada. The present study aims to provide morphological and molecular characterization of three pin nematode species, namely P. neoprojectus, P. tateae, and a new species, Paratylenchus enigmaticus sp. nov. All of them were recovered from the potato growing region of southern Alberta. The nematodes were isolated using the sieving and flotation-centrifugation method, and their morphology was assessed by light microscopy. Molecular characterization was performed using partial 18S, D2–D3 expansion domains of the 28S and ITS ribosomal genes. This study is the first report of molecular characterization of P. tateae and P. neoprojectus, being new records from southern Alberta, and two Spanish populations of P. tateae comprising the first report of this species in Europe. The phylogenetic analysis of the 18S, D2–D3 expansion domains of the 28S and ITS ribosomal DNA regions underscores the importance of using molecular data for accurate species identification and clarifies the status of P. nanus type B and P. sheri. Moreover, our findings will be useful to determine the impact of pin nematodes on potato production in future field research.
dc.description.peer-reviewYes
dc.identifier.citationMunawar, M., Yevtushenko, D. P., Palomares-Rius, J. E., & Castillo, P. (2021). Species diversity of pin nematodes (Paratylenchus spp.) from potato growing regions of southern Alberta, Canada. Plants, 10(2), Article 188. https://doi.org/10.3390/plants10020188
dc.identifier.urihttps://hdl.handle.net/10133/6902
dc.language.isoen
dc.publisherMDPI
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionSpanish National Research Council
dc.publisher.urlhttps://doi.org/10.3390/plants10020188
dc.subjectParatylenchus tateae
dc.subjectParatylenchus neoprojectus
dc.subjectPlant-parasitic nematode
dc.subjectIntegrative taxonomy
dc.subjectMorphology
dc.subjectDNA sequencing
dc.subjectPhylogeny
dc.subjectNew record
dc.subjectNew species
dc.subjectPotato production
dc.subject.lcshNematodes--Alberta, Southern
dc.subject.lcshPotatoes--Alberta, Southern
dc.titleSpecies diversity of pin nematodes (Paratylenchus spp.) from potato growing regions of southern Alberta, Canada
dc.typeArticle
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