Engineering cell-free systems for synthetic biologists

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dc.contributor.authorSheahan, Taylor Anne
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2022-11-07T19:56:06Z
dc.date.available2022-11-07T19:56:06Z
dc.date.issued2022
dc.degree.levelPh.Den_US
dc.description.abstractSynthetic biology (synbio) has emerged as a transformative scientific field with immense potential to address a wide-range of global problems. A specific sub-field of synbio utilizes cellular biomolecular machinery outside of a living cell. In theory, these “cell-free” systems offer a simpler approach and unique features compared to cell-based systems for biotechnology development. However, in practice limited accessibility and poor protein synthesis capacity hinder the overall scope and application of cell-free synbio. To address these challenges, it was our goal to create new engineering tools that will help expand the overall utility of cell-free expression systems. Data presented here provides: 1) detailed methods for the in-house preparation of a cost-effective in vitro reconstituted cell-free system, 2) an in-depth proteomic analysis of the system building blocks as a tool to characterize the composition and inform optimization, and 3) an improvement to protein synthesis capacity by modifying the ribosome composition. Furthermore, a critical assessment of the regulatory landscape is provided, promoting the safe and responsible use of cell-free synbio.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6379
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectsynthetic biologyen_US
dc.subjectcell-free systemsen_US
dc.subjectSynthetic biologyen_US
dc.subjectProteins--Synthesisen_US
dc.subjectProteomicsen_US
dc.subjectBiotechnologyen_US
dc.subjectBioengineeringen_US
dc.subjectDissertations, Academicen_US
dc.titleEngineering cell-free systems for synthetic biologistsen_US
dc.typeThesisen_US
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