Comparative variation within the genome of Campylobacter jejuni NCTC 11168 in human and murine hosts
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Date
2014
Authors
Thomas, Dallas K.
Lone, Abdul G.
Selinger, L. Brent
Taboada, Eduardo N.
Uwiera, Richard R.E.
Abbott, D. Wade
Inglis, G. Douglas
Journal Title
Journal ISSN
Volume Title
Publisher
Public LIbrary of Science
Abstract
Campylobacteriosis incited by
C. jejuni
is a significant enteric disease of human beings. A person working with two reference
strains of
C. jejuni
National Collection of Type Cultures (NCTC) 11168 developed symptoms of severe enteritis including
bloody diarrhea. The worker was determined to be infected by
C. jejuni
. In excess of 50 isolates were recovered from the
worker’s stool. All of the recovered isolates and the two reference strains were indistinguishable from each other based on
comparative genomic fingerprint subtyping. Whole genome sequence analysis indicated that the worker was infected with
a
C. jejuni
NCTC 11168 obtained from the American Type Culture Collection; this strain (NCTC 11168-GSv) is the genome
sequence reference. After passage through the human host, major genetic changes including indel mutations within twelve
contingency loci conferring phase variations were detected in the genome of
C. jejuni
. Specific and robust single nucleotide
polymorphism (SNP) changes in the human host were also observed in two loci (Cj0144c, Cj1564). In mice inoculated with
an isolate of
C. jejuni
NCTC 11168-GSv from the infected person, the isolate underwent further genetic variation. At nine loci,
mutations specific to inoculated mice including five SNP changes were observed. The two predominant SNPs observed in
the human host reverted in mice. Genetic variations occurring in the genome of
C. jejuni
in mice corresponded to increased
densities of
C. jejuni
cells associated with cecal mucosa. In conclusion,
C. jejuni
NCTC 11168-GSv was found to be highly
virulent in a human being inciting severe enteritis. Host-specific mutations in the person with enteritis occurred/were
selected for in the genome of
C. jejuni
, and many were not maintained in mice. Information obtained in the current study
provides new information on host-specific genetic adaptation by
C. jejuni
.
Description
Sherpa Romeo green journal. Open access, distributed under the terms of the Creative Commons Attibution License.
Keywords
Campylobacter , C. jejuni
Citation
Thomas, D.K., Lone, A.G., Selinger, L.B., Taboada, E.N., Uwiera, R.R.E, Abbott, D.W., & Inglis, G.D. (2014). Comparative variation within the genome of Campylobacte jejuni NCTC 11168 in human and murine hosts. PLoS ONE, 9(2), e88229. doi:10.1371/journal/pone.0088229