Comparative variation within the genome of Campylobacter jejuni NCTC 11168 in human and murine hosts

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dc.contributor.authorThomas, Dallas K.
dc.contributor.authorLone, Abdul G.
dc.contributor.authorSelinger, L. Brent
dc.contributor.authorTaboada, Eduardo N.
dc.contributor.authorUwiera, Richard R.E.
dc.contributor.authorAbbott, D. Wade
dc.contributor.authorInglis, G. Douglas
dc.date.accessioned2018-05-31T19:06:58Z
dc.date.available2018-05-31T19:06:58Z
dc.date.issued2014
dc.descriptionSherpa Romeo green journal. Open access, distributed under the terms of the Creative Commons Attibution License.en_US
dc.description.abstractCampylobacteriosis incited by C. jejuni is a significant enteric disease of human beings. A person working with two reference strains of C. jejuni National Collection of Type Cultures (NCTC) 11168 developed symptoms of severe enteritis including bloody diarrhea. The worker was determined to be infected by C. jejuni . In excess of 50 isolates were recovered from the worker’s stool. All of the recovered isolates and the two reference strains were indistinguishable from each other based on comparative genomic fingerprint subtyping. Whole genome sequence analysis indicated that the worker was infected with a C. jejuni NCTC 11168 obtained from the American Type Culture Collection; this strain (NCTC 11168-GSv) is the genome sequence reference. After passage through the human host, major genetic changes including indel mutations within twelve contingency loci conferring phase variations were detected in the genome of C. jejuni . Specific and robust single nucleotide polymorphism (SNP) changes in the human host were also observed in two loci (Cj0144c, Cj1564). In mice inoculated with an isolate of C. jejuni NCTC 11168-GSv from the infected person, the isolate underwent further genetic variation. At nine loci, mutations specific to inoculated mice including five SNP changes were observed. The two predominant SNPs observed in the human host reverted in mice. Genetic variations occurring in the genome of C. jejuni in mice corresponded to increased densities of C. jejuni cells associated with cecal mucosa. In conclusion, C. jejuni NCTC 11168-GSv was found to be highly virulent in a human being inciting severe enteritis. Host-specific mutations in the person with enteritis occurred/were selected for in the genome of C. jejuni , and many were not maintained in mice. Information obtained in the current study provides new information on host-specific genetic adaptation by C. jejuni .en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationThomas, D.K., Lone, A.G., Selinger, L.B., Taboada, E.N., Uwiera, R.R.E, Abbott, D.W., & Inglis, G.D. (2014). Comparative variation within the genome of Campylobacte jejuni NCTC 11168 in human and murine hosts. PLoS ONE, 9(2), e88229. doi:10.1371/journal/pone.0088229en_US
dc.identifier.urihttps://hdl.handle.net/10133/5115
dc.language.isoen_USen_US
dc.publisherPublic LIbrary of Scienceen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionAgriculture and Agri-Food Canada Research Centreen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionPublic Health Agency of Canadaen_US
dc.publisher.institutionUniversity of Albertaen_US
dc.subjectCampylobacteren_US
dc.subjectC. jejunien_US
dc.titleComparative variation within the genome of Campylobacter jejuni NCTC 11168 in human and murine hostsen_US
dc.typeArticleen_US
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