Development of molecular tools to assess whether Arcobacter butzleri is an enteric pathogen of human beings

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dc.contributor.authorWebb, Andrew L.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSelinger, L. Brent
dc.contributor.supervisorInglis, G. Douglas
dc.date.accessioned2016-09-13T21:29:05Z
dc.date.available2016-09-13T21:29:05Z
dc.date.issued2016
dc.degree.levelMastersen_US
dc.description.abstractThe pathogenicity of Arcobacter butzleri remains enigmatic, in part due to a lack of genomic data and tools for comprehensive detection and genotyping of this bacterium. Comparative whole genome sequence analysis was employed to develop a high throughput and high resolution subtyping method representative of whole genome phylogeny. In addition, primers targeting a taxon-specific gene (quinohemoprotein amine dehydrogenase) were designed to detect and quantitate A. butzleri. The application of these methods showed that A. butzleri is present at high frequencies but low densities in diarrheic and healthy people, and specific strains are associated with human enteritis. The developed tools were also used to determine that A. butzleri is common in wastewater, survives tertiary wastewater treatment, and may be transmitted to people via ingestion of contaminated surface water. Diverse subtypes of A. butzleri occur in the environment, but pathogenicity is likely strain-specific and/or dependent on other factors such as host resistance.en_US
dc.description.sponsorshipThis work was supported by the Alberta Livestock and Meat Agency (ALMA) [2012F034R]; and the Government of Canada (Agriculture and Agri-Food Canada [1485]).en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/4606
dc.language.isoen_CAen_US
dc.proquest.subject0307en_US
dc.proquest.subject0766en_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectArcobacter butzlerien_US
dc.subjectwaterborne pathogenen_US
dc.subjectenteric diseaseen_US
dc.titleDevelopment of molecular tools to assess whether Arcobacter butzleri is an enteric pathogen of human beingsen_US
dc.typeThesisen_US
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