The biochemical characterization of Saccharomyces cerevisiae H/ACA small nucleolar ribonucleoproteins

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dc.contributor.authorDurand, Jessica
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden-Kothe, Ute
dc.contributor.supervisorRoussel, Marc R.
dc.date.accessioned2012-07-16T18:18:36Z
dc.date.available2012-07-16T18:18:36Z
dc.date.issued2010
dc.degree.levelMasters
dc.descriptionvii, 83 leaves : ill. ; 29 cmen_US
dc.description.abstractRibosome biogenesis is a crucial yet poorly understood and complex process in all cells. To date, most studies on eukaryotic ribosome biogenesis have relied on yeast genetics and whole cell analysis of ribosomal RNA processing. An early and critical step in ribosome biogenesis is the post-transcriptional modification of rRNA. Pseudouridylation is the most frequently occurring modification. Pseudouridylation is catalyzed by H/ACA small nucleolar ribonucleoproteins (snoRNPs) which are one of the two major classes of snoRNPs found within eukaryotes and archaea. H/ACA snoRNPs consist of four conserved core proteins Cbf5, Gar1, Nop10, and Nhp2 (eukaryotes), and a substrate specific H/ACA snoRNA. Mutations causing the rare inherited disease Dyskeratosis congenita are found in CBF5, NOP10, and NHP2. Here I report the purification of H/ACA protein Cbf5 in the presence of detergents. Additionally, I report initial in vitro RNA binding studies using Nhp2 and the snoRNA snR34 as well as the effects of Dyskeratosis congenita substitutions within Nhp2 on this interaction.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3101
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2010en_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectSaccharomyces cerevisiae -- Researchen_US
dc.subjectNucleoproteins -- Researchen_US
dc.subjectPost-translational modification -- Researchen_US
dc.subjectRibosomes -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleThe biochemical characterization of Saccharomyces cerevisiae H/ACA small nucleolar ribonucleoproteinsen_US
dc.typeThesisen_US
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