The temporal distribution of planktonic protists in southwestern Alberta and their role in the persistence of the human pathogen Campylobacter jejuni

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dc.contributor.authorThomas, Matthew C.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSelinger, L. Brent
dc.contributor.supervisorInglis, G. Douglas
dc.date.accessioned2014-10-29T18:01:39Z
dc.date.available2014-10-29T18:01:39Z
dc.date.issued2013
dc.degree.levelMastersen_US
dc.description.abstractThe temporal dynamics of Campylobacter jejuni and protistan community structure in southwestern Alberta rivers were studied over a 1 -year period. Culture-based isolations of C. jejuni were predominantly from the winter while real-time quantitative PCR indicated fluctuating densities of C. jejuni with no seasonal trend and no correlation with physicochemical properties of water. Protistan communities clustered according to season rather than location. Clinical C. jejuni isolates from southwestern Alberta survived longer when co-cultured with the model protozoan Acanthamaoeba polyphaga than in the presence of by-products or growth medium. The use of wild type and mutant C. jejuni 81-176 strains revealed a cytotoxic effect toward A. polyphaga, and that invasion requires a functional flagellar apparatus but not quorum sensing nor cytolethal distending toxin. Combined, findings illuminate seasonal patterns of C. jejuni and protists and support the hypothesis that C. jejuni exploit phagotrophic protists as a survival mechanism in water. IVen_US
dc.identifier.urihttps://hdl.handle.net/10133/3575
dc.language.isoen_CAen_US
dc.proquest.subject0410en_US
dc.proquest.subject0718en_US
dc.proquest.subject0792en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectcampylobacteriaen_US
dc.subjectaquatic ecosystemsen_US
dc.subjectacanthamoeba polyphagiaen_US
dc.subjectphagotrophic protistsen_US
dc.subjectecologyen_US
dc.subjectenvironmental scienceen_US
dc.titleThe temporal distribution of planktonic protists in southwestern Alberta and their role in the persistence of the human pathogen Campylobacter jejunien_US
dc.typeThesisen_US
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