Genomic and epigenomic responses to chronic stress involve miRNA-mediated programming
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Date
2012
Authors
Babenko, Olena M.
Golubov, Andrey
Ilnytskyy, Yaroslav
Kovalchuk, Igor
Metz, Gerlinde A. S.
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Abstract
Stress represents a critical influence on motor system function and has been shown to impair movement performance. We
hypothesized that stress-induced motor impairments are due to brain-specific changes in miRNA and protein-encoding
gene expression. Here we show a causal link between stress-induced motor impairment and associated genetic and
epigenetic responses in relevant central motor areas in a rat model. Exposure to two weeks of mild restraint stress altered
the expression of 39 genes and nine miRNAs in the cerebellum. In line with persistent behavioural impairments, some
changes in gene and miRNA expression were resistant to recovery from stress. Interestingly, stress up-regulated the
expression of Adipoq and prolactin receptor mRNAs in the cerebellum. Stress also altered the expression of Prlr, miR-186,
and miR-709 in hippocampus and prefrontal cortex. In addition, our findings demonstrate that miR-186 targets the gene
Eps15. Furthermore, we found an age-dependent increase in EphrinB3 and GabaA4 receptors. These data show that even
mild stress results in substantial genomic and epigenomic changes involving miRNA expression and associated gene targets
in the motor system. These findings suggest a central role of miRNA-regulated gene expression in the stress response and in
associated neurological function.
Description
Sherpa Romeo green journal: open access
Keywords
Motor system function , Genomic changes , Eipgenomic changes , miRNA expression , Stress , Gene expression , Motor impairments , Behavioural impairments
Citation
Babenko, O., Golubov, A., Ilnyskyy, Y., Kovalchuk, I., & Metz, G. A. (2012). Genomic and epigenomic responses to chronic stress involve miRNA-mediated programming. PLoS ONE, 7(1): e29441. doi:10.1371/journal.pone.0029441