Development and optimization of efficient regeneration and transformation method for drug-type cultivars of Cannabis sativa

Simple item page
dc.contributor.authorSorokin, Aleksei
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKovalchuk, Igor
dc.contributor.supervisorKovalchuk, Olga
dc.date.accessioned2023-01-25T19:35:53Z
dc.date.available2023-01-25T19:35:53Z
dc.date.issued2022
dc.degree.levelPh.Den_US
dc.description.abstractCannabis has enormous economic importance, considering that all parts of the cannabis plant are used, seeds for food, stem for fiber, and flowers/leaves for medicine. In this research, we have developed tools and techniques that have potential applications to advance cannabis breeding and genetic engineering for germplasm improvement, preservation, and multiplication. Specifically, we developed research methods including rapid and efficient seed germination in sterile conditions, pollen germination assay to assess viability, long-term pollen storage to preserve the germplasm, tissue culture method for mass production of genetically identical plants, and transient expression method to study the gene function.en_US
dc.description.sponsorshipMITACS funding agencyen_US
dc.identifier.urihttps://hdl.handle.net/10133/6421
dc.language.isoen_USen_US
dc.proquest.subject0306en_US
dc.proquest.subject0479en_US
dc.proquest.subject0473en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCannabis sativaen_US
dc.subjectTransient expressionen_US
dc.subjectCryopreservationen_US
dc.subjectRapid germinationen_US
dc.subjectSeed sterilizationen_US
dc.subject.lcshPlant tissue culture
dc.subject.lcshCannabis--Research
dc.subject.lcshGermplasm resources, Plant
dc.subject.lcshGermination
dc.subject.lcshDissertations, Academic
dc.titleDevelopment and optimization of efficient regeneration and transformation method for drug-type cultivars of Cannabis sativaen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ALEKSEI_SOROKIN_PHD_2022.pdf
Size:
6.74 MB
Format:
Adobe Portable Document Format
Description:
Thesis
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
3.25 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Arts and Science, Faculty of
University of Lethbridge Theses