Development and optimization of efficient regeneration and transformation method for drug-type cultivars of Cannabis sativa
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Date
2022
Authors
Sorokin, Aleksei
University of Lethbridge. Faculty of Arts and Science
Journal Title
Journal ISSN
Volume Title
Publisher
Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
Abstract
Cannabis has enormous economic importance, considering that all parts of the cannabis
plant are used, seeds for food, stem for fiber, and flowers/leaves for medicine. In this
research, we have developed tools and techniques that have potential applications to
advance cannabis breeding and genetic engineering for germplasm improvement,
preservation, and multiplication. Specifically, we developed research methods including
rapid and efficient seed germination in sterile conditions, pollen germination assay to
assess viability, long-term pollen storage to preserve the germplasm, tissue culture method
for mass production of genetically identical plants, and transient expression method to
study the gene function.
Description
Keywords
Cannabis sativa , Transient expression , Cryopreservation , Rapid germination , Seed sterilization