Tandem RNA affinity purifications to study an essential snoRNA in ribosome assembly

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dc.contributor.authorRocca, Daniel G.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKothe, Ute
dc.contributor.supervisorRoussel, Marc R.
dc.date.accessioned2023-01-20T18:39:42Z
dc.date.available2023-01-20T18:39:42Z
dc.date.issued2022
dc.degree.levelMastersen_US
dc.description.abstractEukaryotic ribosome assembly is an essential process involving many protein and RNA assembly factors. Mutations of genes encoding ribosomal proteins or assembly factors can result in diseases in humans. The upregulation of ribosome assembly is also a hallmark of many cancers. snR30 is an interesting assembly factor due to its role as an unusual and essential H/ACA small nucleolar RNA and as it is one of only three essential RNA assembly factors. Here, I have developed a system for the co-expression of aptamer-tagged variants of snR30 and truncations of ribosomal RNA in Saccharomyces cerevisiae. Next, I optimized the tandem purifications of the snR30-bound pre-ribosomes utilizing these aptamers. In conclusion, my system will allow for in-depth characterization of the composition and structure of snR30-bound ribosome precursors. This characterization will improve the understanding of the essential activity of snR30 for ribosome assembly in yeast and inform future studies on the function of the homologous U17 RNA in humans.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6414
dc.language.isoen_CAen_US
dc.proquest.subject0487en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectsnR30
dc.subjectRibosome assembly
dc.subject.lcshRNA
dc.subject.lcshRibosomes
dc.subject.lcshBiochemistry
dc.subject.lcshBioengineering
dc.subject.lcshDissertations, Academic
dc.titleTandem RNA affinity purifications to study an essential snoRNA in ribosome assemblyen_US
dc.typeThesisen_US
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