Establishing and optimizing a pipeline for using a combined multi-omic approach to assessing the role of eIF5B in glioblastoma cells

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dc.contributor.authorVanden Dungen, Keiran John
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorThakor, Nehal
dc.date.accessioned2021-02-08T20:13:01Z
dc.date.available2021-02-08T20:13:01Z
dc.date.issued2020
dc.degree.levelMastersen_US
dc.description.abstractTranslation is an important and highly regulated process. Specifically, translation initiation is of interest when considering the ways in which cells respond to treatments and environmental changes. Previously, we demonstrated that eIF5B is responsible for the translation of a subset of mRNAs encoding anti-apoptotic proteins which regulated the sensitivity of U343’s resistance to TRAIL. Through this project, I have established an important multi-omics pipeline for investigating the impact of eIF5B on glioblastoma cells. By utilizing this pipeline, I have demonstrated that both TRAIL and eIF5B individually affected the glioblastoma cell metabolome, and in combination revealed further changes. Specifically, these treatment conditions produced robust shifts in metabolites such as myo-inositol. I have successfully prepared sequencing-ready RNA libraries for Ribo-seq with corresponding RNA for RNA-seq. This research will provide our lab the opportunity to expand my research on eIF5B through this pipeline as I have demonstrated that eIF5B-modulated metabolites are very cancer-relevant.en_US
dc.identifier.urihttps://hdl.handle.net/10133/5838
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0410en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectMetabolomicsen_US
dc.subjectTranslatomicsen_US
dc.subjectTranscriptomicsen_US
dc.subjectGlioblastomaen_US
dc.subjecteIF5Ben_US
dc.subjectTumor necrosis factor -- Researchen_US
dc.subjectGenetic translation -- Regulation -- Researchen_US
dc.subjectGlioblastoma multiforme -- Growth -- Regulation -- Researchen_US
dc.subjectMetabolites -- Researchen_US
dc.subjectApoptosis -- Researchen_US
dc.subjectGuanosine triphosphatase -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleEstablishing and optimizing a pipeline for using a combined multi-omic approach to assessing the role of eIF5B in glioblastoma cellsen_US
dc.typeThesisen_US
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