Developmental and reproductive effects of 2-(2H-Benzotriazol-2-yl)-4-methylphenol (UV-P) on the model fish species, zebrafish (Danio rerio), following embryonic exposure

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dc.contributor.authorRaza, Yamin
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWiseman, Steve
dc.date.accessioned2024-01-05T20:43:24Z
dc.date.available2024-01-05T20:43:24Z
dc.date.issued2023
dc.degree.levelMasters
dc.description.abstractBenzotriazole ultraviolet stabilizers (BUVSs) are emerging contaminants of concern. They are added to a variety of products including building materials, personal care products, and paints to prevent degradation caused by UV light. Despite widespread occurrence in aquatic environments, little is known regarding effects of BUVSs on aquatic organisms. The aim of this study was to characterize effects of exposure to 2-(2H-Benzotriazol-2-yl)-4-methylphenol (UV-P) on reproductive success of adult zebrafish (Danio rerio) following embryonic exposure. Zebrafish embryos were exposed, by use of microinjection, to UV-P at a control, low, and high dose, then reared until sexual maturity, where reproductive performance was assessed, following which molecular and biochemical endpoints were analyzed. Exposure to UV-P did not have a significant effect on fecundity. However, there was a significant effect on fertilization success. Using UV-P exposed males and females, fertility was decreased in the low and high treatments, relative to control. Additionally, in a reproduction assay with UV-P exposed males and control females, fertility was decreased in the high treatment, relative to the control. Embryonic exposure to UV-P might have perturbed male sex steroid synthesis as indicated by small changes in blood plasma concentrations of E2 and 11-KT, and small decreases in mRNA abundances of cyp19a1a, cyp11c1, and hsd17b3. Additionally, decreased transcript abundances of genes involved in spermatogenesis was observed. Decreases in later stages of sperm development were observed, suggesting that embryonic exposure to UV-P impaired spematogenesis, resulting in decreased sperm quantity. This study is the first to demonstrate latent effects of BUVSs, specifically on fish reproduction.
dc.identifier.urihttps://hdl.handle.net/10133/6651
dc.language.isoen
dc.proquest.subject0306
dc.proquest.subject0409
dc.proquest.subject0307
dc.proquestyesYes
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbrige. Faculty of Arts and Science)
dc.subjectreproductive toxicity
dc.subjectendocrine disruption
dc.subjectspermatogenesis
dc.subjectlatent effects
dc.subjectbenzotriazole UV stabilizer
dc.subjectfertilization
dc.subject.lcshReproductive toxicology--Research
dc.subject.lcshEndocrine disrupting chemicals in water--Research
dc.subject.lcshSpermatogenesis in animals
dc.subject.lcshFertilization (Biology)
dc.subject.lcshReproductive health--Environmental aspects
dc.subject.lcshEndocrine toxicology--Research
dc.subject.lcshEnvironmental toxicology--Research
dc.subject.lcshZebra danio--Effect of chemicals on--Research
dc.subject.lcshZebra danio--Reproduction--Research
dc.subject.lcshZebra danios as laboratory animals
dc.subject.lcshStabilizing agents
dc.subject.lcshUltraviolet radiation
dc.subject.lcshDissertations, Academic
dc.subject.lcshEmerging contaminants in water--Research
dc.titleDevelopmental and reproductive effects of 2-(2H-Benzotriazol-2-yl)-4-methylphenol (UV-P) on the model fish species, zebrafish (Danio rerio), following embryonic exposure
dc.typeThesis
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