Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli 0157:H7 super-shedders
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Date
2016
Authors
Wang, Ou
Liang, Guanxiang
McAllister, Tim A.
Plastow, Graham
Stanford, Kim
Selinger, L. Brent
Guan, Le Luo
Journal Title
Journal ISSN
Volume Title
Publisher
Public LIbrary of Science
Abstract
Super-shedder cattle are a major disseminator of
E
.
coli
O157:H7 into the environment, and
the terminal rectum has been proposed as the primary
E
.
coli
O157:H7 colonization site.
This study aimed to identify host factors that are associated with the super-shedding pro-
cess by comparing transcriptomic profiles in rectal tissue collected from 5 super-shedder
cattle and 4 non-shedder cattle using RNA-Seq. In total, 17,859
±
354 genes and 399
±
16
miRNAs were detected, and 11,773 genes were expressed in all animals. Fifty-eight differ-
entially expressed (DE) genes (false discovery rate
<
0.05) including 11 up-regulated and
47 down-regulated (log 2 (fold change) ranged from -5.5 to 4.2), and 2 up-regulated DE
miRNAs (log 2 (fold change) = 2.1 and 2.5, respectively) were identified in super-shedders
compared to non-shedders. Functional analysis of DE genes revealed that 31 down-regu-
lated genes were potentially associated with reduced innate and adaptive immune functions
in super-shedders, including 13 lymphocytes membrane receptors, 3 transcription factors
and 5 cytokines, suggesting the decreased key host immune functions in the rectal tissue of
super-shedders, including decreased quantity and migration of immune cells such as lym-
phocytes, neutrophils and dendritic cells. The up-regulation of bta-miR-29d-3p and the
down regulation of its predicted target gene,
regulator of G-protein signaling 13
, suggested
a potential regulatory role of this miRNA in decreased migration of lymphocytes in super-
shedders. Based on these findings, the rectal tissue of super-shedders may inherently
exhibit less effective innate and adaptive immune protection. Further study is required to
confirm if such effect on host immunity is due to the nature of the host itself or due to actions
mediated by
E
.
coli
O157:H7.
Description
Sherpa Romeo green journal. Open access, distributed under the terms of the Creative Commons Attribution License
Keywords
E-coli 0157:H7 , Super-shedder cattle , Rectal tissue , Immunity , Host factors , Transcriptomic profiles
Citation
Want, O., Liang, G., McAllister, T.A., Plastow, G., Stanford, K., Selinger, B., & Guan, L.L. (2016). Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli 0157:H7 super-shedders. PLoS ONE, 11(3), e0151284. doi:10.1371/journal.pone.0151284