Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli 0157:H7 super-shedders

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dc.contributor.authorWang, Ou
dc.contributor.authorLiang, Guanxiang
dc.contributor.authorMcAllister, Tim A.
dc.contributor.authorPlastow, Graham
dc.contributor.authorStanford, Kim
dc.contributor.authorSelinger, L. Brent
dc.contributor.authorGuan, Le Luo
dc.date.accessioned2018-05-31T20:10:29Z
dc.date.available2018-05-31T20:10:29Z
dc.date.issued2016
dc.descriptionSherpa Romeo green journal. Open access, distributed under the terms of the Creative Commons Attribution Licenseen_US
dc.description.abstractSuper-shedder cattle are a major disseminator of E . coli O157:H7 into the environment, and the terminal rectum has been proposed as the primary E . coli O157:H7 colonization site. This study aimed to identify host factors that are associated with the super-shedding pro- cess by comparing transcriptomic profiles in rectal tissue collected from 5 super-shedder cattle and 4 non-shedder cattle using RNA-Seq. In total, 17,859 ± 354 genes and 399 ± 16 miRNAs were detected, and 11,773 genes were expressed in all animals. Fifty-eight differ- entially expressed (DE) genes (false discovery rate < 0.05) including 11 up-regulated and 47 down-regulated (log 2 (fold change) ranged from -5.5 to 4.2), and 2 up-regulated DE miRNAs (log 2 (fold change) = 2.1 and 2.5, respectively) were identified in super-shedders compared to non-shedders. Functional analysis of DE genes revealed that 31 down-regu- lated genes were potentially associated with reduced innate and adaptive immune functions in super-shedders, including 13 lymphocytes membrane receptors, 3 transcription factors and 5 cytokines, suggesting the decreased key host immune functions in the rectal tissue of super-shedders, including decreased quantity and migration of immune cells such as lym- phocytes, neutrophils and dendritic cells. The up-regulation of bta-miR-29d-3p and the down regulation of its predicted target gene, regulator of G-protein signaling 13 , suggested a potential regulatory role of this miRNA in decreased migration of lymphocytes in super- shedders. Based on these findings, the rectal tissue of super-shedders may inherently exhibit less effective innate and adaptive immune protection. Further study is required to confirm if such effect on host immunity is due to the nature of the host itself or due to actions mediated by E . coli O157:H7.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationWant, O., Liang, G., McAllister, T.A., Plastow, G., Stanford, K., Selinger, B., & Guan, L.L. (2016). Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli 0157:H7 super-shedders. PLoS ONE, 11(3), e0151284. doi:10.1371/journal.pone.0151284en_US
dc.identifier.urihttps://hdl.handle.net/10133/5116
dc.language.isoen_USen_US
dc.publisherPublic LIbrary of Scienceen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Albertaen_US
dc.publisher.institutionLethbridge Research Centreen_US
dc.publisher.institutionAlberta Agriculture and Forestryen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectE-coli 0157:H7en_US
dc.subjectSuper-shedder cattleen_US
dc.subjectRectal tissueen_US
dc.subjectImmunityen_US
dc.subjectHost factorsen_US
dc.subjectTranscriptomic profilesen_US
dc.titleComparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli 0157:H7 super-sheddersen_US
dc.typeArticleen_US
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