Nanoscale structure determination of Murray Valley encephalitis and Powassan virus non-coding RNAs

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dc.contributor.authorMrozowich, Tyler
dc.contributor.authorHenrickson, Amy
dc.contributor.authorDemeler, Borries
dc.contributor.authorPatel, Trushar R.
dc.date.accessioned2021-07-02T19:16:02Z
dc.date.available2021-07-02T19:16:02Z
dc.date.issued2020
dc.descriptionOpen access article. Creative Commons Attribution 4.0 International License (CC BY 4.0) appliesen_US
dc.description.abstractViral infections are responsible for numerous deaths worldwide. Flaviviruses, which contain RNA as their genetic material, are one of the most pathogenic families of viruses. There is an increasing amount of evidence suggesting that their 5’ and 3’ non-coding terminal regions are critical for their survival. Information on their structural features is essential to gain detailed insights into their functions and interactions with host proteins. In this study, the 5’ and 3’ terminal regions of Murray Valley encephalitis virus and Powassan virus were examined using biophysical and computational modeling methods. First, we used size exclusion chromatography and analytical ultracentrifuge methods to investigate the purity of in-vitro transcribed RNAs. Next, we employed small-angle X-ray scattering techniques to study solution conformation and low-resolution structures of these RNAs,which suggest that the 3’ terminal regions are highly extended as compared to the 5’ terminal regions for both viruses. Using computational modeling tools, we reconstructed 3-dimensional structures of each RNA fragment and compared them with derived small-angle X-ray scattering low-resolution structures. This approach allowed us to reinforce that the 5’ terminal regions adopt more dynamic structures compared to the mainly double-stranded structures of the 3’ terminal regions.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationMrozowich, T., Henrickson, A., Demeler, B., & Patel, T. R. (2020). Nanoscale structure determination of Murray Valley encephalitis and Powassan virus non-coding RNAs. Viruses, 12(2), Article 190. https://doi.org/10.3390/v12020190en_US
dc.identifier.urihttps://hdl.handle.net/10133/5937
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionUniversity of Montanaen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.institutionUniversity of Albertaen_US
dc.publisher.urlhttps://doi.org/10.3390/v12020190en_US
dc.subjectNon-coding RNAen_US
dc.subjectMurray Valley encephalitis virusen_US
dc.subjectPowwassan virusen_US
dc.subjectFlavivirusen_US
dc.subjectAnalytical ultracentrifugationen_US
dc.subjectSmall-angle X-ray scatteringen_US
dc.subjectComputational RNAen_US
dc.titleNanoscale structure determination of Murray Valley encephalitis and Powassan virus non-coding RNAsen_US
dc.typeArticleen_US
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