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dc.contributor.author Nshogozabahizi, J. C.
dc.contributor.author Aubrey, K. L.
dc.contributor.author Ross, J. A.
dc.contributor.author Thakor, Nehal
dc.date.accessioned 2020-10-23T22:34:49Z
dc.date.available 2020-10-23T22:34:49Z
dc.date.issued 2019
dc.identifier.citation Nshogozabahizi, J. C., Aubrey, K. L., Ross, J. A., & Thakor, N. (2019). Applications and limitations of regulatory RNA elements in synthetic biology and biotechnology. Journal of Applied Microbiology, 127(4), 968-984. https://doi.org/10.1111/jam.14270 en_US
dc.identifier.uri https://hdl.handle.net/10133/5795
dc.description This is the peer reviewed version of this article. It may be used for non-commercial purposed in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. en_US
dc.description.abstract Synthetic biology requires the design and implementation of novel enzymes, genetic circuits or even entire cells, which can be controlled by the user. RNA‐based regulatory elements have many important functional properties in this regard, such as their modular nature and their ability to respond to specific external stimuli. These properties have led to the widespread exploration of their use as gene regulation devices in synthetic biology. In this review, we focus on two major types of RNA elements: riboswitches and RNA thermometers (RNATs). We describe their general structure and function, before discussing their potential uses in synthetic biology (e.g. in the production of biofuels and biodegradable plastics). We also discuss their limitations, and novel strategies to implement RNA‐based regulatory devices in biotechnological applications. We close with a description of some common model organisms used in synthetic biology, with a focus on the current applications and limitations of RNA‐based regulation. en_US
dc.language.iso en_US en_US
dc.publisher Wiley en_US
dc.subject Biotechnology en_US
dc.subject Cyanophyta en_US
dc.subject Ralstonia sp. en_US
dc.subject Riboswitches en_US
dc.subject RNA elements en_US
dc.subject RNA thermometers (RNATs) en_US
dc.subject Synthetic biology en_US
dc.subject.lcsh Cyanobacteria
dc.title Applications and limitations of regulatory RNA elements in synthetic biology and biotechnology en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Lethbridge en_US
dc.publisher.url https://doi.org/10.1111/jam.14270 en_US


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