Applications and limitations of regulatory RNA elements in synthetic biology and biotechnology

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dc.contributor.authorNshogozabahizi, J. C.
dc.contributor.authorAubrey, K. L.
dc.contributor.authorRoss, J. A.
dc.contributor.authorThakor, Nehal
dc.date.accessioned2020-10-23T22:34:49Z
dc.date.available2020-10-23T22:34:49Z
dc.date.issued2019
dc.descriptionThis is the peer reviewed version of this article. It may be used for non-commercial purposed in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en_US
dc.description.abstractSynthetic biology requires the design and implementation of novel enzymes, genetic circuits or even entire cells, which can be controlled by the user. RNA‐based regulatory elements have many important functional properties in this regard, such as their modular nature and their ability to respond to specific external stimuli. These properties have led to the widespread exploration of their use as gene regulation devices in synthetic biology. In this review, we focus on two major types of RNA elements: riboswitches and RNA thermometers (RNATs). We describe their general structure and function, before discussing their potential uses in synthetic biology (e.g. in the production of biofuels and biodegradable plastics). We also discuss their limitations, and novel strategies to implement RNA‐based regulatory devices in biotechnological applications. We close with a description of some common model organisms used in synthetic biology, with a focus on the current applications and limitations of RNA‐based regulation.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationNshogozabahizi, J. C., Aubrey, K. L., Ross, J. A., & Thakor, N. (2019). Applications and limitations of regulatory RNA elements in synthetic biology and biotechnology. Journal of Applied Microbiology, 127(4), 968-984. https://doi.org/10.1111/jam.14270en_US
dc.identifier.urihttps://hdl.handle.net/10133/5795
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://doi.org/10.1111/jam.14270en_US
dc.subjectBiotechnologyen_US
dc.subjectCyanophytaen_US
dc.subjectRalstonia sp.en_US
dc.subjectRiboswitchesen_US
dc.subjectRNA elementsen_US
dc.subjectRNA thermometers (RNATs)en_US
dc.subjectSynthetic biologyen_US
dc.subject.lcshCyanobacteria
dc.titleApplications and limitations of regulatory RNA elements in synthetic biology and biotechnologyen_US
dc.typeArticleen_US
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