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dc.contributor.supervisor Thakor, Nehal
dc.contributor.author Bressler, Kamiko R.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2020-01-17T18:00:25Z
dc.date.available 2020-01-17T18:00:25Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10133/5666
dc.description.abstract Cap-dependent translation drives the global synthesis of proteins. Under stress conditions global protein production is attenuated, yet the translation of ATF4 is upregulated through uORF- mediated translation initiation. eIF5B has been shown to deliver initiator-tRNA during non-canonical translation initiation. As such, we defined the role of eIF5B in the non-canonical translation of ATF4, and p27. Through polysome profiling and luciferase reporter assays, we confirmed that eIF5B facilitates uORF2-mediated repression of ATF4 translation. We determined that eIF5B has transcriptome-wide effects on signaling pathways, verifying activation of the JNK arm of the MAPK pathway and upregulation of dyskerin. I investigated the role eIF5B has in regulation of p27, and suggest that the mechanism is IRES-dependent. This study furthers the understanding into mechanisms of alternative translation initiation, which is critical to gene expression regulation. en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science)
dc.subject Messenger RNA
dc.subject Proteins--Synthesis
dc.subject Ribosomes
dc.title Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry & Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject Biochemistry [0487] en_US
dc.proquest.subject Cellular biology [0379] en_US
dc.proquest.subject Molecular biology [0307] en_US
dc.proquestyes Yes en_US


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