Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation

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dc.contributor.authorBressler, Kamiko R.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorThakor, Nehal
dc.date.accessioned2020-01-17T18:00:25Z
dc.date.available2020-01-17T18:00:25Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractCap-dependent translation drives the global synthesis of proteins. Under stress conditions global protein production is attenuated, yet the translation of ATF4 is upregulated through uORF- mediated translation initiation. eIF5B has been shown to deliver initiator-tRNA during non-canonical translation initiation. As such, we defined the role of eIF5B in the non-canonical translation of ATF4, and p27. Through polysome profiling and luciferase reporter assays, we confirmed that eIF5B facilitates uORF2-mediated repression of ATF4 translation. We determined that eIF5B has transcriptome-wide effects on signaling pathways, verifying activation of the JNK arm of the MAPK pathway and upregulation of dyskerin. I investigated the role eIF5B has in regulation of p27, and suggest that the mechanism is IRES-dependent. This study furthers the understanding into mechanisms of alternative translation initiation, which is critical to gene expression regulation.en_US
dc.identifier.urihttps://hdl.handle.net/10133/5666
dc.language.isoen_CAen_US
dc.proquest.subjectBiochemistry [0487]en_US
dc.proquest.subjectCellular biology [0379]en_US
dc.proquest.subjectMolecular biology [0307]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry & Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectMessenger RNA
dc.subjectProteins--Synthesis
dc.subjectRibosomes
dc.titleElucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiationen_US
dc.typeThesisen_US
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