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dc.contributor.supervisor Wieden, Hans-Joachim
dc.contributor.author Girodat, Dylan J.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2019-08-14T15:33:44Z
dc.date.available 2019-08-14T15:33:44Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10133/5493
dc.description.abstract Guanosine triphosphatases (GTPases) are some of the most vital and versatile molecular switches found in all domains of life. For proper functionality these enzymes require allosteric regulation mediated by ligand binding. This thesis highlights three key problems in allosteric regulation: equilibrium, transmission, and transition, investigating how GTPases address each problem. The free energy equilibrium upon nucleotide binding to Elongation Factor (EF)-Tu was investigated to describe how GTPases tackle the equilibrium problem of allostery. Additionally, the mechanism of how the ribosome allosterically regulates the GTPase activity of EF-Tu was investigated to describe how GTPases can solve the transmission problem of allostery. Lastly, the D2 dopamine receptor conformational ensemble revealed how the transition problem of allostery can be addressed in guanosine nucleotide exchange factors. Altogether this thesis provides a framework which can be used to study the three problems of allostery in any protein, highlighting strategies utilized by GTPases. en_US
dc.description.sponsorship Alberta Innovates (Strategic Chairs Program [SC60-T2] Natural Sciences and Engineering Research Discovery Grant [RGPIN-2016-05199] Alberta Innovates Technology Futures Graduate Student Scholarship en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Guanosine triphosphatase -- Research en_US
dc.subject Allosteric regulation -- Research en_US
dc.subject Enzymes -- Regulation -- Research en_US
dc.subject allosteric regulation en_US
dc.subject allostery en_US
dc.subject EF-Tu en_US
dc.subject GTPases en_US
dc.subject guanosine triphosphatases en_US
dc.subject molecular switches en_US
dc.subject Dissertations, Academic en_US
dc.title Allosteric regulation and function of GTPases en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Ph.D en_US
dc.proquest.subject 0786 en_US
dc.proquest.subject 0487 en_US
dc.proquest.subject 0307 en_US
dc.proquestyes Yes en_US


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