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dc.contributor.supervisor Mosimann, Steven
dc.contributor.supervisor Selinger, L. Brent
dc.contributor.author Cleland, Colyn
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2019-07-22T17:22:55Z
dc.date.available 2019-07-22T17:22:55Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10133/5478
dc.description.abstract The X-ray crystallographic structure of a divergent PTPLP from Desulfovibrio magneticus has been determined in the presence and absence of InsP6 substrate in order to identify the structural features that give rise to its 3-4-5 substrate specificity. These include a novel Phy loop conformation and R242 of the HCRGG.GR P-loop signature sequence. Further, PTPLPs containing an arginine following the cysteine nucleophile are expected to preferentially target the C4-phosphoryl of Ins(1,2,4,5,6)P5 and have a 3-4 hydrolytic pathway. Subsequently, both InsP6 and Ins(1,2,6)P3 were covalently attached to chromatographic resin and utilized as ’bait’ molecules in novel affinity pull-down experiments utilizing a phosphate control column and unfractionated whole cell lysates. The resulting interactomes contain a greater fraction of known IP interacting proteins than current literature methods and identify similar or larger number of total proteins. Despite these improvements, more work needs to be done to address non-specific binding to our columns. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry & Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject protein complex en_US
dc.subject substrate specificity en_US
dc.subject PTP-like en_US
dc.subject affinity pull-down en_US
dc.subject Cysteine proteinases en_US
dc.subject Inositol phosphates en_US
dc.subject Phosphatases en_US
dc.subject X-ray crystallography en_US
dc.title Structure and function of PhyA from Desulfovibrio magneticus and use of its product in affinity pull-down experiments en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry & Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject Molecular biology [0307] en_US
dc.proquest.subject Biochemistry [0487] en_US
dc.proquest.subject Molecular physics [0609] en_US
dc.proquestyes Yes en_US


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