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dc.contributor.supervisor Wieden-Kothe, Ute
dc.contributor.author Ogailan, Abeer Abdullah
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2017-10-16T16:57:27Z
dc.date.available 2017-10-16T16:57:27Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/10133/4933
dc.description.abstract The effect that Dyskeratosis congenita mutations have on ribosome biogenesis has not been fully investigated. Here, I am studying the effect of Dyskeratosis congenita mutations using a yeast model system with the goal of identifying impacts on ribosome biogenesis. I constructed six haploid yeast strains with a single mutation in the CBF5 gene each using site-directed mutagenesis and chromosomal integration. First, I screened for phenotypic effects on growth on solid and in liquid medium at different temperatures; these experiments show normal growth behavior of the mutant strains compared to wild type. Second, Cbf5p expression levels were examined in whole-cell extract via western blotting analysis, which again did not reveal any difference to wild type. Finally, progression through the cell cycle was investigated in synchronized cells. Together, this study indicates that individual Dyskeratosis congenita mutations are well tolerated in the unicellular eukaryote Saccharomyces cerevisiae. en_US
dc.description.sponsorship KING ABDULLAH BIN ABDULAZIZ SCHOLARSHIP en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta : University of Lethbridge, Dept. of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject CBF5 gene en_US
dc.subject Dyskeratosis congenita mutations en_US
dc.subject H/ACA RNA en_US
dc.subject H/ACA sRNP en_US
dc.subject ribosome biogenesis en_US
dc.subject yeast strains en_US
dc.title Construction and initial characterization of yeast strains with Dyskeratosis congenita mutations en_US
dc.type Thesis en_US
dc.publisher.faculty Art and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0487 en_US
dc.proquestyes Yes en_US
dc.embargo No en_US


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