Construction and initial characterization of yeast strains with Dyskeratosis congenita mutations

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dc.contributor.authorOgailan, Abeer Abdullah
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden-Kothe, Ute
dc.date.accessioned2017-10-16T16:57:27Z
dc.date.available2017-10-16T16:57:27Z
dc.date.issued2017
dc.degree.levelMastersen_US
dc.description.abstractThe effect that Dyskeratosis congenita mutations have on ribosome biogenesis has not been fully investigated. Here, I am studying the effect of Dyskeratosis congenita mutations using a yeast model system with the goal of identifying impacts on ribosome biogenesis. I constructed six haploid yeast strains with a single mutation in the CBF5 gene each using site-directed mutagenesis and chromosomal integration. First, I screened for phenotypic effects on growth on solid and in liquid medium at different temperatures; these experiments show normal growth behavior of the mutant strains compared to wild type. Second, Cbf5p expression levels were examined in whole-cell extract via western blotting analysis, which again did not reveal any difference to wild type. Finally, progression through the cell cycle was investigated in synchronized cells. Together, this study indicates that individual Dyskeratosis congenita mutations are well tolerated in the unicellular eukaryote Saccharomyces cerevisiae.en_US
dc.description.sponsorshipKING ABDULLAH BIN ABDULAZIZ SCHOLARSHIPen_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/4933
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArt and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCBF5 geneen_US
dc.subjectDyskeratosis congenita mutationsen_US
dc.subjectH/ACA RNAen_US
dc.subjectH/ACA sRNPen_US
dc.subjectribosome biogenesisen_US
dc.subjectyeast strainsen_US
dc.titleConstruction and initial characterization of yeast strains with Dyskeratosis congenita mutationsen_US
dc.typeThesisen_US
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DYSKERATOSIS CONGENITA mutation, western blotting,growth analysis, flow cytometery
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