Show simple item record Kovalchuk, Igor Walz, Paul Thomas, James E. Kovalchuk, Olga 2016-10-31T18:17:54Z 2016-10-31T18:17:54Z 2013
dc.identifier.citation Kovalchuk, I., Walz, P., Thomas, J., & Kovalchuk, O. (2013). Genomic instability in liver cells caused by an LPS-induced bystander-like effect en_US
dc.description Sherpa Romeo green journal: open access en_US
dc.description.abstract Bacterial infection has been linked to carcinogenesis, however, there is lack of knowledge of molecular mechanisms that associate infection with the development of cancer. We analyzed possible effects of the consumption of heat-killed E. coli O157:H7 cells or its cellular components, DNA, RNA, protein or lipopolysaccharides (LPS) on gene expression in naı¨ve liver cells. Four week old mice were provided water supplemented with whole heat-killed bacteria or bacterial components for a two week period. One group of animals was sacrificed immediately, whereas another group was allowed to consume uncontaminated tap water for an additional two weeks, and liver samples were collected, post mortem. Liver cells responded to exposure of whole heat-killed bacteria and LPS with alteration in cH2AX levels and levels of proteins involved in proliferation, DNA methylation (MeCP2, DNMT1, DNMT3A and 3B) or DNA repair (APE1 and KU70) as well as with changes in the expression of genes involved in stress response, cell cycle control and bile acid biosynthesis. Other bacterial components analysed in this study did not lead to any significant changes in the tested molecular parameters. This study suggests that lipopolysaccharides are a major component of Gram-negative bacteria that induce molecular changes within naı¨ve cells of the host. en_US
dc.language.iso en_CA en_US
dc.publisher Public Library of Science en_US
dc.subject Liver cells en_US
dc.subject Lipopolysaccharides en_US
dc.subject LPS en_US
dc.subject Gene expression en_US
dc.subject Bacterial infection en_US
dc.subject Genome instability en_US
dc.subject Bystander effect en_US
dc.title Genomic instability in liver cells caused by an LPS-induced bystander-like effect en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Lethbridge en_US

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