Show simple item record Boyko, Alexander Blevins, Todd Yao, Youli Golubov, Andrey Bilichak, Andriy Ilnytskyy, Yaroslav Hollunder, Jens Meins, Frederick Kovalchuk, Igor 2016-10-27T19:00:04Z 2016-10-27T19:00:04Z 2010
dc.identifier.citation Boyko, A., Blevins, T., Yao, Y., Golubov, A., Bilichak, A., Ilnytskyy, Y., ... Kovalchuk, I. (2010). Transgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of dicer-like proteins. PLoS ONE, 5(3), e9514. doi:10.1371/journal.pone.0009514 en_US
dc.description Sherpa Romeo green journal: open access en_US
dc.description.abstract Epigenetic states and certain environmental responses in mammals and seed plants can persist in the next sexual generation. These transgenerational effects have potential adaptative significance as well as medical and agronomic ramifications. Recent evidence suggests that some abiotic and biotic stress responses of plants are transgenerational. For example, viral infection of tobacco plants and exposure of Arabidopsis thaliana plants to UVC and flagellin can induce transgenerational increases in homologous recombination frequency (HRF). Here we show that exposure of Arabidopsis plants to stresses, including salt, UVC, cold, heat and flood, resulted in a higher HRF, increased global genome methylation, and higher tolerance to stress in the untreated progeny. This transgenerational effect did not, however, persist in successive generations. Treatment of the progeny of stressed plants with 5-azacytidine was shown to decrease global genomic methylation and enhance stress tolerance. Dicer-like (DCL) 2 and DCL3 encode Dicer activities important for small RNAdependent gene silencing. Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants, while in dcl2 mutants, only stress-induced stress tolerance was impaired. Our results are consistent with the hypothesis that stress-induced transgenerational responses in Arabidopsis depend on altered DNA methylation and smRNA silencing pathways. en_US
dc.language.iso en_CA en_US
dc.publisher Public Library of Science en_US
dc.subject Arabidopsis thaliana en_US
dc.subject Stressed plants en_US
dc.subject Progeny en_US
dc.subject Transgenerational responses en_US
dc.subject DNA methylation en_US
dc.subject Stress tolerance en_US
dc.title Transgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of dicer-like proteins en_US
dc.type Article en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.description.peer-review Yes en_US
dc.publisher.institution University of Lethbridge en_US
dc.publisher.institution Friedrich Miescher Institute for Biomedical Research en_US
dc.publisher.institution Ghent University en_US

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