Transgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of dicer-like proteins

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dc.contributor.authorBoyko, Alexander
dc.contributor.authorBlevins, Todd
dc.contributor.authorYao, Youli
dc.contributor.authorGolubov, Andrey
dc.contributor.authorBilichak, Andriy
dc.contributor.authorIlnytskyy, Yaroslav
dc.contributor.authorHollunder, Jens
dc.contributor.authorMeins, Frederick
dc.contributor.authorKovalchuk, Igor
dc.date.accessioned2016-10-27T19:00:04Z
dc.date.available2016-10-27T19:00:04Z
dc.date.issued2010
dc.descriptionSherpa Romeo green journal: open accessen_US
dc.description.abstractEpigenetic states and certain environmental responses in mammals and seed plants can persist in the next sexual generation. These transgenerational effects have potential adaptative significance as well as medical and agronomic ramifications. Recent evidence suggests that some abiotic and biotic stress responses of plants are transgenerational. For example, viral infection of tobacco plants and exposure of Arabidopsis thaliana plants to UVC and flagellin can induce transgenerational increases in homologous recombination frequency (HRF). Here we show that exposure of Arabidopsis plants to stresses, including salt, UVC, cold, heat and flood, resulted in a higher HRF, increased global genome methylation, and higher tolerance to stress in the untreated progeny. This transgenerational effect did not, however, persist in successive generations. Treatment of the progeny of stressed plants with 5-azacytidine was shown to decrease global genomic methylation and enhance stress tolerance. Dicer-like (DCL) 2 and DCL3 encode Dicer activities important for small RNAdependent gene silencing. Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants, while in dcl2 mutants, only stress-induced stress tolerance was impaired. Our results are consistent with the hypothesis that stress-induced transgenerational responses in Arabidopsis depend on altered DNA methylation and smRNA silencing pathways.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationBoyko, A., Blevins, T., Yao, Y., Golubov, A., Bilichak, A., Ilnytskyy, Y., ... Kovalchuk, I. (2010). Transgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of dicer-like proteins. PLoS ONE, 5(3), e9514. doi:10.1371/journal.pone.0009514en_US
dc.identifier.urihttps://hdl.handle.net/10133/4636
dc.language.isoen_CAen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionFriedrich Miescher Institute for Biomedical Researchen_US
dc.publisher.institutionGhent Universityen_US
dc.subjectArabidopsis thalianaen_US
dc.subjectStressed plantsen_US
dc.subjectProgenyen_US
dc.subjectTransgenerational responsesen_US
dc.subjectDNA methylationen_US
dc.subjectStress toleranceen_US
dc.titleTransgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of dicer-like proteinsen_US
dc.typeArticleen_US
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