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dc.contributor.supervisor Ziemienowicz, Alicja
dc.contributor.supervisor Kovalchuk, Igor
dc.contributor.author Matsuoka, Aki
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2016-09-13T18:54:44Z
dc.date.available 2016-09-13T18:54:44Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/10133/4603
dc.description.abstract Agrobacterium tumefaciens has been adopted to become a greatest tool for plant genetic engineering. Since Agrobacteria predominantly integrate single copy of the transgene into the nuclear genome, the transgene expression is relatively low. In contrast, transgene expression from chloroplasts allows high expression. Therefore, my first objective was to develop a new method of chloroplast transformation by modifying Agrobacterium genetics. I attempted to transform tobacco chloroplasts by Agrobacterium carrying the VirD2 protein with inactivated nuclear localization signals (NLSs) fused to a chloroplast transit peptide. However, the transformation of tobacco chloroplasts was unsuccessful. It suggested that the transit peptide was overridden by a novel cryptic NLS of VirD2. My second objective was to determine a novel NLS in VirD2. I discovered the novel NLS in VirD2 by subcellular localization analyses of fusion proteins (fragment of VirD2-green fluorescent protein) in plant cells. My discovery contributed to characterization of VirD2. en_US
dc.description.sponsorship Alberta Innovates Technology Futures en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta : University of Lethbridge, Dept. of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Agrobacterium-mediated transformation en_US
dc.subject Chloroplast transformation en_US
dc.subject VirD2 en_US
dc.subject Nuclear localization signals en_US
dc.subject Transit peptides en_US
dc.subject Protein diffusion through the nuclear pore en_US
dc.subject Subcellular localization en_US
dc.subject Protein subcellular localization in leaves en_US
dc.title Genetic engineering of Agrobacterium tumefaciens to target chloroplasts and identification of a novel nuclear localization signal of VirD2 protein en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Ph.D en_US
dc.proquest.subject 0307 en_US
dc.proquestyes Yes en_US
dc.embargo.start 2015-09
dc.embargo.end 2016-02-29
dc.embargo Yes en_US


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