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dc.contributor.supervisor Schultz, Elizabeth
dc.contributor.author Pahari, Shankar
dc.date.accessioned 2013-12-10T22:23:17Z
dc.date.available 2013-12-10T22:23:17Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/10133/3337
dc.description xv, 100 leaves : ill. ; 29 cm en_US
dc.description.abstract Asymmetric localization of PIN proteins controls directionality of auxin transport and many aspects of plant development. The Arabidopsis mutant, unhinged-1 (unh-1), has defects to leaf veins and other root and shoot phenotypes. I identify UNH as the Arabidopsis VPS51 homologue, a member of the Arabidopsis GARP complex, and show that UNH interacts with VPS52, another member of the complex. I also show that UNH co-localizes with SYP61, a trans Golgi network marker. The GARP complex in yeast and metazoans retrieves vacuolar sorting receptors to the TGN and is important in sorting proteins for lysosomal degradation. PIN1 expression in the margin of unh-1 leaves is expanded and the unh leaf phenotype is suppressed by pin1 mutation, supporting the idea that the phenotype results from expanded PIN expression. My results suggest the UNH is important to restrict expression of PIN1 within the margin, likely by targeting PIN1 to the lytic vacuole. en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, [Dept. of] Biological Sciences, c2012 en_US
dc.subject Plants -- Effect of auxin on en_US
dc.subject Auxin en_US
dc.subject Arabidopsis en_US
dc.title Unhinged encodes a VPS51 homologue of Arabidopsis and reveals a role for the GARP complex in leaf shape and vein patterning en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US


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