Subunit interactions within box C/D sRNPs
Janzen, Timothy William
University of Lethbridge. Faculty of Arts and Science
Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2010
Box C/D small ribonucleoproteins (box C/D sRNPs) are responsible for the 2’-O-methylation required for the complete maturation of precursor rRNA. Archaeal box C/D sRNPs, like eucarya, are composed of four components: a guide RNA (box C/D sRNA), an RNA binding protein (L7ae), a 2’-O-methyltransferase (Fibrillarin) and a structural protein (Nop5). Here we develop several approaches for studying box C/D sRNP assembly. In particular, we have used pulldown and mobility shift assays to identify box C/D sRNP assembly intermediates (Nop5-aFib and L7ae-sR1). We have also demonstrated that isothermal titration calorimetry (ITC) can be utilized to quantitatively characterize the energetics of formation for the L7ae-sRNA assembly intermediate.
xi, 98 leaves : ill. (some col.) ; 29 cm
Nucleoproteins , RNA , Methylation , Archaebacteria -- Molecular aspects , Dissertations, Academic