Subunit interactions within box C/D sRNPs
| dc.contributor.author | Janzen, Timothy William | |
| dc.contributor.author | University of Lethbridge. Faculty of Arts and Science | |
| dc.contributor.supervisor | Mosimann, Steven C. | |
| dc.date.accessioned | 2011-10-30T21:07:22Z | |
| dc.date.available | 2011-10-30T21:07:22Z | |
| dc.date.issued | 2010 | |
| dc.degree.level | Masters | |
| dc.description | xi, 98 leaves : ill. (some col.) ; 29 cm | en_US |
| dc.description.abstract | Box C/D small ribonucleoproteins (box C/D sRNPs) are responsible for the 2’-O-methylation required for the complete maturation of precursor rRNA. Archaeal box C/D sRNPs, like eucarya, are composed of four components: a guide RNA (box C/D sRNA), an RNA binding protein (L7ae), a 2’-O-methyltransferase (Fibrillarin) and a structural protein (Nop5). Here we develop several approaches for studying box C/D sRNP assembly. In particular, we have used pulldown and mobility shift assays to identify box C/D sRNP assembly intermediates (Nop5-aFib and L7ae-sR1). We have also demonstrated that isothermal titration calorimetry (ITC) can be utilized to quantitatively characterize the energetics of formation for the L7ae-sRNA assembly intermediate. | en_US |
| dc.identifier.uri | https://hdl.handle.net/10133/2547 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2010 | en_US |
| dc.publisher.department | Department of Chemistry and Biochemistry | en_US |
| dc.publisher.faculty | Arts and Science | en_US |
| dc.relation.ispartofseries | Thesis (University of Lethbridge. Faculty of Arts and Science) | en_US |
| dc.subject | Nucleoproteins | en_US |
| dc.subject | RNA | en_US |
| dc.subject | Methylation | en_US |
| dc.subject | Archaebacteria -- Molecular aspects | en_US |
| dc.subject | Dissertations, Academic | en_US |
| dc.title | Subunit interactions within box C/D sRNPs | en_US |
| dc.type | Thesis | en_US |