Genomic and epigenomic changes in the progeny of cold-stressed Arabidopsis thaliana plants

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dc.contributor.authorRahman, Ashif
dc.contributor.authorYadav, Narendra Singh
dc.contributor.authorByeon, Boseon
dc.contributor.authorIlnytskyy, Yaroslav
dc.contributor.authorKovalchuk, Igor
dc.date.accessioned2024-10-31T21:12:19Z
dc.date.available2024-10-31T21:12:19Z
dc.date.issued2024
dc.descriptionOpen access article. Creative Commons Attribution International 4.0 license (CC BY 4.0) applies
dc.description.abstractPlants are continuously exposed to various environmental stresses. Because they can not escape stress, they have to develop mechanisms of remembering stress exposures somatically and passing it to the progeny. We studied the Arabidopsis thaliana ecotype Columbia plants exposed to cold stress for 25 continuous generations. Our study revealed that multigenerational exposure to cold stress resulted in the changes in the genome and epigenome (DNA methylation) across generations. Main changes in the progeny were due to the high frequency of genetic mutations rather than epigenetic changes; the difference was primarily in single nucleotide substitutions and deletions. The progeny of cold-stressed plants exhibited the higher rate of missense non-synonymous mutations as compared to the progeny of control plants. At the same time, epigenetic changes were more common in the CHG (C = cytosine, H = cytosine, adenine or thymine, G = guanine) and CHH contexts and favored hypomethylation. There was an increase in the frequency of C to T (thymine) transitions at the CHH positions in the progeny of cold stressed plants; because this type of mutations is often due to the deamination of the methylated cytosines, it can be hypothesized that environment-induced changes in methylation contribute to mutagenesis and may be to microevolution processes and that RNA-dependent DNA methylation plays a crucial role. Our work supports the existence of heritable stress response in plants and demonstrates that genetic changes prevail.
dc.description.peer-reviewYes
dc.identifier.citationRahman, A., Yadav, N. S., Byeon, B., Ilnytskyy, Y., & Kovalchuk, I. (2024). Genomic and epigenomic changes in the progeny of cold-stressed Arabidopsis thaliana plants. International Journal of Molecular Sciences, 25(5), Article 2795. https://doi.org/10.3390/ijms25052795
dc.identifier.urihttps://hdl.handle.net/10133/6945
dc.language.isoen
dc.publisherMDPI
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionState University of New York
dc.publisher.urlhttps://doi.org/10.3390/ijms25052795
dc.subjectMultigenerational cold stress
dc.subjectStress memory
dc.subjectArabidopsis thaliana
dc.subjectIntergenerational inheritance
dc.subjectEpigenetic variations
dc.subjectGenetic variations
dc.subjectSNPs
dc.subjectInDels
dc.subject.lcshPlants--Effect of cold on
dc.subject.lcshCold--Physiological effect
dc.subject.lcshPlant genetics
dc.subject.lcshEpigenetics
dc.subject.lcshPlants--Effect of stress on
dc.titleGenomic and epigenomic changes in the progeny of cold-stressed Arabidopsis thaliana plants
dc.typeArticle
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