Prevalence of Shiga-toxin producing Escherichia coli in Alberta beef cattle and characteristics of non-pathogenic microcin producing Escherichia coli champion battling bacterial virulence

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dc.contributor.authorPaquette, Sarah-Jo
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorReuter, Tim
dc.contributor.supervisorThomas, James E.
dc.date.accessioned2020-05-07T17:20:42Z
dc.date.available2020-05-07T17:20:42Z
dc.date.issued2020
dc.degree.levelPh.Den_US
dc.description.abstractZoonotic pathogens, like Shiga toxin-producing Escherichia coli (STEC) are a food safety and health risk. Among STEC, O157 is considered a major pathogen; however, globally the number of outbreaks with new emerging STEC strains is rising. Here, quantitative data on STEC O157 were gathered for western-Canada beef cattle and compared to emerging STEC. Quantitative data revealed; O178 is 4-times more numerous than O157 and high Shiga-toxin events are often not attributed to O157. Results suggest current surveillance likely misses emerging STEC due to restricted O-serogroup screening. Secondly, an alternate STEC mitigation strategy was investigated as an alternative approach to use of antibiotics which is controversial in human and animal therapeutics. Competition experiments identified a strong E. coli O103F which incapacitated STEC growth by producing a diffusible antimicrobial, most likely a microcin. The antimicrobial revealed tremendous potential for use along the farm-to-fork continuum or in human intervention to mitigate STEC.en_US
dc.identifier.urihttps://hdl.handle.net/10133/5715
dc.language.isoen_USen_US
dc.proquest.subjectMicrobiology [0410]en_US
dc.proquest.subjectMolecular biology [0307]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectBeef -- Albertaen_US
dc.subjectCattle -- Albertaen_US
dc.subjectEscherichia colien_US
dc.subjectEscherichia coli infectionsen_US
dc.subjectEscherichia coli infections in animalsen_US
dc.subjectEscherichia coli O157:H7en_US
dc.titlePrevalence of Shiga-toxin producing Escherichia coli in Alberta beef cattle and characteristics of non-pathogenic microcin producing Escherichia coli champion battling bacterial virulenceen_US
dc.typeThesisen_US
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