Radiation-induced deregulation of PiRNA pathway proteins : a possible molecular mechanism underlying transgenerational epigenomic instability

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dc.contributor.authorMerrifield, Matthew
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKovalchuk, Olga
dc.date.accessioned2012-03-01T18:22:15Z
dc.date.available2012-03-01T18:22:15Z
dc.date.issued2011
dc.degree.levelMasters
dc.descriptionix, 123 leaves : ill. (some col.) ; 29 cmen_US
dc.description.abstractPiRNAs and their Piwi family protein partners are part of a germline specific epigenetic regulatory mechanism essential for proper spermatogenesis, silencing of transposable elements, and maintaining germline genome integrity, yet their role in the response of the male germline to genotoxic stress is unknown. Ionizing radiation (IR) is known to cause transgenerational genome instability that is linked to carcinogenesis. Although the molecular etiology of IR-induced transgenerational genomic instability is not fully understood, it is believed to be an epigenetically mediated phenomenon. IR-induced alterations in the expression pattern of key regulatory proteins involved in the piRNA pathway essential for paternal germline genome stability may be directly involved in producing epigenetic alterations that can impact future generations. Here we show whole body and localized X-irradiation leads to significant altered expression of proteins that are necessary for, and intimately involved in, the proper functioning of the germline specific piRNA pathway in mice and rats. In addition we found that IR-induced alterations to piRNA pathway protein levels were time and dose dependent.en_US
dc.identifier.urihttps://hdl.handle.net/10133/2617
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Science, c2011en_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectPiwi genes -- Effect of radiation on -- Researchen_US
dc.subjectIonizing radiation -- Researchen_US
dc.subjectGenes -- Effect of radiation on -- Researchen_US
dc.subjectGerm cells -- Effect of radiation on -- Researchen_US
dc.subjectGenetic toxicology -- Researchen_US
dc.subjectMice as laboratory animalsen_US
dc.subjectRats as laboratory animalsen_US
dc.subjectDissertations, Academicen_US
dc.titleRadiation-induced deregulation of PiRNA pathway proteins : a possible molecular mechanism underlying transgenerational epigenomic instabilityen_US
dc.typeThesisen_US
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