The rpsA gene and its product ribosomal protein S1: investigating the role of RNA structure in translation

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dc.contributor.authorHeller, Jalyce L.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2020-01-22T16:46:13Z
dc.date.available2020-01-22T16:46:13Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractThe rpsA gene contains a translation initiation region (TIR) which lacks a Shine-Dalgarno element, is highly structured, and which efficiently drives translation in bacteria. The rpsA gene is downregulated by its gene product, ribosomal protein S1. To investigate how the structure of the TIR facilitates function both in translation and downregulation, we employed a combined SAXS, SHAPE, and computational approach. The structure-function relationship reveals that the introduction of single nucleotide substitutions in the TIR alters its structure, corresponding to a modulated translation efficiency in vivo. The activity of the rpsA TIR in a minimal in vitro transcription/translation system was investigated to understand the TIR’s requirement for additional factors. We observed that S1 is strictly required for its translation, and that S1 specifically recognizes and binds to the TIR when in excess over ribosomes. The structure-driven mechanism of this TIR may represent a previously overlooked strategy of translation initiation in bacteria.en_US
dc.description.sponsorshipAlberta Innovates (Strategic Chairs Program [SC60-T2], Natural Sciences and Engineering Research Council of Canada Graduate Scholarship- Mastersen_US
dc.identifier.urihttps://hdl.handle.net/10133/5669
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0786en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectRibosomal protein S1en_US
dc.subjectTranslationen_US
dc.subjectrpsAen_US
dc.subjectbacteriaen_US
dc.subjecttranslation initiation regionen_US
dc.subjectDissertations, Academicen_US
dc.titleThe rpsA gene and its product ribosomal protein S1: investigating the role of RNA structure in translationen_US
dc.typeThesisen_US
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