Epigenetic inheritance and transgenerational response to heat stress in Arabidopsis thaliana

dc.contributor.authorAyemere, Ivie
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKovalchuk, Igor
dc.date.accessioned2020-01-14T16:54:09Z
dc.date.available2020-01-14T16:54:09Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractPlants experience abiotic stress and develop mechanisms to tolerate these stresses which preserves their progeny over time. Stress exposure induces physiological responses like accelerated growth rates; increased flowering/bolting time complementary to altered levels of genomic methylation. To understand the effects of epigenetic inheritance in the form of DNA methylation, changes triggered by heat stress in progeny genome, epigenetic variations in the genome of stressed and non-stressed progeny and parent plants over twenty-five generations were compared using whole genome bisulfite sequencing. Our study provides evidence that multigenerational exposure to heat stress resulted in physiological changes and impacted DNA methylation patterns across generations. Identification of SNPs gave insight to the broader genotypic diversity in stressed plants and showed a pattern of inheritance that reflects short-term memory of heat stress across twenty-five generations in Arabidopsis thaliana. Understanding transgenerational responses in plants can assist in implementing new techniques that produce non-transgenic stress tolerant crops.en_US
dc.identifier.urihttps://hdl.handle.net/10133/5659
dc.language.isoen_USen_US
dc.proquest.subjectMolecular biology [0307]en_US
dc.proquest.subjectBotany [0309]en_US
dc.proquest.subjectBiology [0306]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectArabidopsis thalianaen_US
dc.subjectEpigeneticsen_US
dc.subjectHeat -- Physiological effecten_US
dc.subjectMethylationen_US
dc.subjectMolecular biologyen_US
dc.titleEpigenetic inheritance and transgenerational response to heat stress in Arabidopsis thalianaen_US
dc.typeThesisen_US
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