Characterization of checkpoint adaptation in human fibroblastic glioma cells and an analysis of protein phosphatase inhibitors

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dc.contributor.authorLanser, Brittany
dc.contributor.supervisorGolsteyn, Roy
dc.date.accessioned2014-04-07T18:38:35Z
dc.date.available2014-04-07T18:38:35Z
dc.date.issued2012
dc.degree.levelMasters
dc.descriptionxi, 114 leaves : ill. (some col.) ; 29 cmen_US
dc.description.abstractThis thesis reports that checkpoint adaptation occurs in human brain cancer cells. M059K cells, after treatment with camptothecin (CPT), recruited γ-histone H2AX, phosphorylated Chk1 and arrested in the G2 phase. Strikingly, cells escaped the checkpoint, became rounded and entered mitosis as measured by phospho-histone H3 signals. Lamin A/C immunofluorescence microscopy revealed that 48% of the cells that survived checkpoint adaptation contained micronuclei. These data suggest that brain cancer cells undergo checkpoint adaptation and may have an altered genome. This thesis also explored if phosphatases participate in checkpoint adaptation. Human colon cancer cells were treated with CPT and the PP2A inhibitor cantharidin. Following treatment the cells became rounded and 65% were positive for phospho-histone H3 signals indicating that cantharidin caused cells to be in mitosis following CPT treatment. These data suggest that PP2A might have a role in checkpoint adaptation, or participate in a pathway that bypasses checkpoint adaptation.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3390
dc.language.isoen_CAen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2012en_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.subjectGliomasen_US
dc.subjectCancer cells -- Adaptationen_US
dc.subjectBrain -- Tumorsen_US
dc.subjectCellular control mechanismsen_US
dc.subjectCell cycle -- Regulationen_US
dc.subjectPhosphoprotein phosphatasesen_US
dc.subjectDissertations, Academicen_US
dc.titleCharacterization of checkpoint adaptation in human fibroblastic glioma cells and an analysis of protein phosphatase inhibitorsen_US
dc.typeThesisen_US
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