Plant organelle targeting cell penetrating peptides

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dc.contributor.authorMacMillan, Trevor
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorEudes, François
dc.contributor.supervisorKovalchuk, Igor
dc.date.accessioned2014-10-17T21:23:13Z
dc.date.available2014-10-17T21:23:13Z
dc.date.issued2014
dc.degree.levelPh.Den_US
dc.degree.levelMasters
dc.descriptionxii, 131 leaves : illustrations (chiefly coloured) ; 29 cmen_US
dc.description.abstractTo address the limitations of conventional nuclear transformation I have developed a peptide based gene delivery system to genetically manipulate the genomes of plant cell mitochondria and plastids. Plant organelle targeting cell penetrating peptides (POTCPPs) are peptides that form nanoparticles composed of short peptides and double-stranded DNA (dsDNA). The peptides have cell penetrating properties and specific organelle targeting properties. These properties enabled the peptides to translocate their cargo across the cell wall and outer plasma membrane of tissue cultured plant cells to deliver dsDNA to the mitochondria or the chloroplast and have it expressed in organello. The POTCPP transfection method represents the first report of a successful in vivo plant cell mitochondrial transfection and a peptide based plastid transfection within a monocot species. The POTCPP transfection method can be applied to organelle gene targeting, functional genomic and to the study of transient gene expression in tissue cultured plant cells.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3543
dc.language.isoen_CAen_US
dc.proquest.subject0307en_US
dc.proquest.subject0479en_US
dc.proquest.subject0539en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectMitochondria transfectionen_US
dc.subjectPlastid transfectionen_US
dc.subjectCell penetrating peptideen_US
dc.subjectChloroplast targeting peptideen_US
dc.subjectPlant cellen_US
dc.subjectPlant mitochondria -- Research
dc.subjectPlant cell biotechnology -- Research
dc.subjectPlastids -- Research
dc.subjectPeptides -- Research
dc.subjectGene targeting -- Research
dc.subjectPlant organelles -- Research
dc.subjectPlant cell membranes -- Research
dc.subjectPlant genetic engineering -- Research
dc.subjectDissertations, Academic
dc.titlePlant organelle targeting cell penetrating peptidesen_US
dc.typeThesisen_US
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