Multigenerational effects of the novel brominated flame retardant 1,2,5,6- tetrabromocyclooctane on reproductive performance in Japanese medaka (Oryzias latipes)

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dc.contributor.authorDevoy, Chloe Elizabeth
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWiseman, Steve
dc.date.accessioned2023-07-21T15:48:27Z
dc.date.available2023-07-21T15:48:27Z
dc.date.issued2023
dc.degree.levelMasters
dc.description.abstractThe brominated flame retardant, 1,2,5,6-tetrabromocyclooctane (TBCO), is an endocrine disrupting chemical (EDC) that’s usage is predicted to increase. It is not known if early-life stage exposure of fish to TBCO causes reproductive impairments, as is the case when sexually mature fish are exposed to this chemical. This thesis demonstrates that TBCO is maternally transferred in Japanese medaka (Oryzias latipes), causing multigenerational reproductive impairment. Following parental (F0) exposure, F1 fecundity was decreased by 26.0-56.8%. Plasma concentrations of 17β-estradiol were not significantly decreased. A small decrease in transcript abundance of vitellogenin was observed, but the magnitude was unlikely to have decreased fecundity. However, ex vivo maturation of oocytes from the F1 generation was significantly decreased by 20.0-23.4%, and transcript abundances of genes that regulate oocyte maturation were consistently decreased. This is the first study to demonstrate that early-life stage exposure to an EDC caused decreased fecundity via inhibition of oocyte maturation.
dc.identifier.urihttps://hdl.handle.net/10133/6542
dc.language.isoen
dc.proquest.subject0768
dc.proquest.subject0306
dc.proquest.subject0409
dc.proquestyesYes
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectendocrine disrupting chemical
dc.subjectmultigenerational
dc.subjectreproduction
dc.subjectoogenesis
dc.subjectJapanese medaka
dc.subjectbrominated flame retardant
dc.subjectoocyte maturation
dc.subject.lcshEndocrine disrupting chemicals--Research
dc.subject.lcshEndocrine disrupting chemicals--Health aspects--Research
dc.subject.lcshEndocrine disrupting chemicals--Environmental aspects--Research
dc.subject.lcshOogenesis
dc.subject.lcshOryzias latipes--Effect of chemicals on--Research
dc.subject.lcshOryzias latipes--Fertility--Effect of chemicals on--Research
dc.subject.lcshOryzias latipes--Reproduction--Effect of chemicals on--Research
dc.subject.lcshOryzias latipes--Life cycles--Effect of chemicals on--Research
dc.subject.lcshFireproofing agents--Toxicology--Research
dc.subject.lcshFish as laboratory animals
dc.subject.lcshOrganobromine compounds--Research
dc.subject.lcshDissertations, Academic
dc.titleMultigenerational effects of the novel brominated flame retardant 1,2,5,6- tetrabromocyclooctane on reproductive performance in Japanese medaka (Oryzias latipes)
dc.typeThesis
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