Anti-mitotic activity of six Asteraceae plant extracts and identification of the sesquiterpene lactone hymenoratin from the Canadian plant Hymenoxys richardsonii

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dc.contributor.authorMolina, Layla
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorGolsteyn, Roy
dc.date.accessioned2019-03-07T23:30:59Z
dc.date.available2019-03-07T23:30:59Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractIn this thesis we investigate the anti-mitotic activity of six Canadian Asteraceae species. Three plant extracts arrested cancer cells in mitosis with different characteristics. Hymenoxys richardsonii extract induced a mitotic arrest measured by rounded cell morphology, phosphorylated histone H3, active Cdk1/Cyclin B, high levels of Plk1, and G2/M arrest by flow cytometry. Mitotic-arrested cells were characterized by chromosome congression failure, distorted mitotic spindles, and damaged DNA. Biology-guided fractionation led to the purification of the active compound hymenoratin, a sesquiterpene lactone closely related to pulchelloid A, which was isolated from the anti-mitotic plant Gaillardia aristata. This is the first time that H. richardsonii or hymenoratin is reported to elicit a biological activity on cancer cells. Investigation of the structure-activity relationship between sesquiterpene lactones and anti-mitotic activity is potentially useful to understand unknown biological mechanisms or to develop anticancer drug candidates.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5298
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0307en_US
dc.proquest.subject0309en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectAntimitotic agents -- Researchen_US
dc.subjectCompositae -- Researchen_US
dc.subjectPlant extracts -- Researchen_US
dc.subjectPlant extracts -- Therapeutic use -- Researchen_US
dc.subjectSesquiterpene lactones -- Researchen_US
dc.subjectCancer cells -- Growth -- Regulation -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.subjectanti-mitotic propertiesen_US
dc.subjectGaillardia aristataen_US
dc.subjecthymenoratinen_US
dc.subjectHymenoxys richardsoniien_US
dc.subjectmitotic arresten_US
dc.titleAnti-mitotic activity of six Asteraceae plant extracts and identification of the sesquiterpene lactone hymenoratin from the Canadian plant Hymenoxys richardsoniien_US
dc.typeThesisen_US
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