The biophysical characterization of the α-carboxysome and a minimal carboxysome

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dc.contributor.authorTurton, Kristi L.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2023-02-27T22:17:59Z
dc.date.available2023-02-27T22:17:59Z
dc.date.issued2022
dc.degree.levelMastersen_US
dc.description.abstractCells use organelles, micelles, and proteinaceous structures to compartmentalize cellular processes. Compartmentalization has the benefit of providing metabolic control, flux, and protective storage of biomaterials. Carboxysomes are proteinaceous compartments that enclose proteins and metabolites involved in the metabolism of carbon dioxide. Due to the nature and biophysical properties of the carboxysome, use in biotechnology applications is being pursued. Using biophysical characterization methods such as size exclusion chromatography-Multi-Angle Light Scattering (SEC-MALS), Transmission Electron Microscopy (TEM), and Analytical Ultracentrifugation (AUC), structural information of wild type carboxysomes expressed in E. coli and carboxysomes engineered to have a minimal set of proteins, known as the minimal carboxysome are obtained. AUC and TEM provided detail with respect to size and mass distributions of individual particles. Data in this thesis indicate the need for further optimization of existing carboxysome purification strategies.en_US
dc.description.sponsorshipNSERC CREATE: RNA Innovation Program; Mitacs Accelerate: Lab2Marketen_US
dc.identifier.urihttps://hdl.handle.net/10133/6438
dc.language.isoen_USen_US
dc.proquest.subject0487
dc.proquest.subject0786
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry & Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectα-carboxysomeen_US
dc.subjectbiophysical characterizationen_US
dc.subjectbiophysical methodsen_US
dc.subjectCell compartmentation--Research
dc.subjectMetabolism
dc.subjectCell physiology
dc.subjectCarbon dioxide--Metabolism
dc.subjectBiotechnology--Research
dc.subjectDissertations
dc.subjectAcademic
dc.titleThe biophysical characterization of the α-carboxysome and a minimal carboxysomeen_US
dc.typeThesisen_US
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