Stochastic modeling of eukaryotic transcription at the single nucleotide level

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dc.contributor.authorVashishtha, Saurabh
dc.contributor.supervisorRoussel, Marc R.
dc.date.accessioned2012-10-22T17:30:24Z
dc.date.available2012-10-22T17:30:24Z
dc.date.issued2011
dc.degree.levelMasters
dc.descriptionx, 107 leaves : ill. ; 29 cmen_US
dc.description.abstractDNA is the genetic material of a cell and is copied in the form of pre-mRNA through transcription in eukaryotes. RNA polymerase II is responsible for the transcription of all genes that express proteins. Transcription is a significant source of the stochasticity in gene expression. In this thesis, I discuss the development of a biochemically detailed model of eukaryotic transcription, which includes pre-initiation complex (PIC) assembly, abortive initiation, promoter-proximal pausing and termination as the points that can be slow steps for transcription. The stochastic properties of this model are studied in detail by stochastic simulations with some preliminary mathematical analysis. The results of this model suggest that PIC assembly can play the most significant role in affecting the transcription dynamics. In addition, promoter-proximal pausing has been identified as a potential noise regulatory step in eukaryotic transcription. These results show excellent agreement with many experimental studies.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3190
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2011en_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectEukaryotic cells -- Researchen_US
dc.subjectGenetic transcription -- Researchen_US
dc.subjectRNA poymerases -- Researchen_US
dc.subjectStochastic modelsen_US
dc.subjectNucleotidesen_US
dc.titleStochastic modeling of eukaryotic transcription at the single nucleotide levelen_US
dc.typeThesisen_US
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