Biophysical characterization of honey bee virus untranslated regions

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dc.contributor.authorLetain, Jenna M.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2025-01-16T18:35:13Z
dc.date.available2025-01-16T18:35:13Z
dc.date.issued2024
dc.degree.levelMasters
dc.description.abstractOver the past two decades, honey bee colony losses have become a serious and widespread issue. Many factors contribute to these losses, but one major driver is viruses. Sacbrood virus and deformed wing virus are found globally and can cause detrimental effects to honey bee colonies. These viruses have a long, single-stranded genome of ribonucleic acid (RNA) flanked by structured untranslated regions. Although noncoding, these regions play important roles in the viral life cycle. While untranslated regions have been studied extensively in other viruses, there is a research gap for honey bee viruses. This thesis focuses on the biophysical characterization of the untranslated regions of these two common honey bee viruses. Small-angle X-ray scattering and computational modelling elucidated low and high-resolution three-dimensional structures for the viral untranslated regions. Functional assays revealed that the sacbrood virus 5′ untranslated region acts as an internal ribosome entry site capable of initiating translation. Furthermore, immunoprecipitation pull-down assays identified potential RNA-binding proteins that interact with the sacbrood virus untranslated regions. Altogether, this thesis provides foundational insights into the characteristics of the untranslated regions of sacbrood virus and deformed wing virus, guiding future research in this field.
dc.embargoNo
dc.identifier.urihttps://hdl.handle.net/10133/6969
dc.language.isoen
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectbiophysical characterization
dc.subjecthoney bee
dc.subjectRNA-binding proteins
dc.subjectbiochemistry
dc.subjectviral RNA
dc.subject.lcshDissertations, Academic
dc.subject.lcshHoneybee--Viruses--Research
dc.subject.lcshHoneybee--Virus diseases--Research
dc.subject.lcshRNA-protein interactions--Research
dc.subject.lcshViruses--Reproduction--Research
dc.subject.lcshNon-coding RNA--Research
dc.subject.lcshPicornaviruses--Research
dc.titleBiophysical characterization of honey bee virus untranslated regions
dc.typeThesis
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