Characterization of NfGHX : a founding member of a new glycoside hydrolase family from the anaerobic fungus Neocallimastix frontalis

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dc.contributor.authorUddin, Muhammed Salah
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbott, D. Wade
dc.contributor.supervisorSelinger, L. Brent
dc.date.accessioned2017-07-17T17:19:57Z
dc.date.available2017-07-17T17:19:57Z
dc.date.issued2016
dc.degree.levelMastersen_US
dc.description.abstractThe discovery and characterization of enzymes that catalyze new reactions, and increase the rate or efficiency of bioconversion are promising lines of biotechnological research. In this regard the rumen anaerobic fungi represent an underexploited source of novel carbohydrate active enzymes that are active on recalcitrant plant cell wall polysaccharides. In this study, four rumen fungal genes (Nf2152, Nf2215, Nf2523 and Pr2455) were identified as functional CAZymes that represent founding members of a new GH family, referred to here as ‘GHX’. Two members of GHX, Nf2152 and Nf2523, were functionally characterized, and it was discovered that they have two different activities. Nf2152 releases a single product (β-1,2-arabinobiose) from sugar beet arabinan (SBA), and β-1,2-arabinobiose and a galactoarabinose with an unknown linkage from rye arabinoxylan (RAX). Nf2523 exclusively produces galactoarabinose from RAX. To the best of my knowledge, this represents the first reported galactoarabinobiosidase. Both β-1,2-arabinobiose and galactoarabinose are not conventional structures within SBA and RAX, and in fact are more likely released from the glycan side chains of arabinogalactan proteins (AGPs) that co-purify with the polysaccharides. In this regard, the GHXs studied here represent valuable tools for sequencing diverse AGP glycans and assisting in their bioconversion for industrial applications, such as animal production.en_US
dc.embargoYesen_US
dc.embargo.end2017-03-01
dc.embargo.start2016-09-01
dc.identifier.urihttps://hdl.handle.net/10133/4877
dc.language.isoen_USen_US
dc.proquest.subject0306en_US
dc.proquest.subject0307en_US
dc.proquest.subject0473en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectarabinoseen_US
dc.subjectenzyme discoveryen_US
dc.subjectgalactoseen_US
dc.subjectglycoside hydrolaseen_US
dc.subjectrumen fungien_US
dc.titleCharacterization of NfGHX : a founding member of a new glycoside hydrolase family from the anaerobic fungus Neocallimastix frontalisen_US
dc.typeThesisen_US
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