A novel anti-mitotic activity on human cells by Pulchelloid A, a compound isolated from the prairie plant Gaillardia aristata

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dc.contributor.authorBosco, Alessandra
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorGolsteyn, Roy
dc.date.accessioned2017-05-23T21:03:24Z
dc.date.available2017-05-23T21:03:24Z
dc.date.issued2017
dc.degree.levelMastersen_US
dc.description.abstractIn this thesis we investigate the prairie plant species Gaillardia aristata for compounds of biological interest. Through the use of phenotypic screens based upon the hallmarks of cancer, we have characterized the effects of G. aristata extracts upon human cell lines, and found that extracts induced mitotic arrest. This arrest was characterized by low levels of pTyr15-Cdk1, and high levels of cyclin B, securin, and pThr320-PP1Cα, indicating that cells arrested at metaphase-anaphase transition. Arrested cells exhibited distorted mitotic spindles and damaged DNA. By biology-guided fractionation, we isolated a compound from G. aristata plant extracts that inhibits mitosis in human cells. The compound was the sesquiterpene lactone Pulchelloid A, and we are the first to identify its activities upon human cells. Pulchelloid A affects mitosis through a mechanism that could involve the inhibition of the ubiquitin-proteasome pathway. Insight into this mitotic process might be beneficial to future studies in cancer therapies.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/4849
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0306en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectanti-cancer propertiesen_US
dc.subjectbioactive secondary metabolitesen_US
dc.subjectblanket floweren_US
dc.subjectmitosis inhibitorsen_US
dc.subjectnative prairie plant medicinal propertiesen_US
dc.subjectsesquiterpene lactoneen_US
dc.subjectCompositae -- Therapeutic use -- Research -- Albertaen_US
dc.subjectEndemic plants -- Therapeutic use -- Research -- Albertaen_US
dc.subjectSesquiterpene lactones -- Research -- Albertaen_US
dc.subjectCancer cells -- Growth -- Regulation -- Researchen_US
dc.subjectMitosis -- Regulation -- Researchen_US
dc.subjectMateria medica, Vegetableen_US
dc.subjectPharmacognosyen_US
dc.titleA novel anti-mitotic activity on human cells by Pulchelloid A, a compound isolated from the prairie plant Gaillardia aristataen_US
dc.typeThesisen_US
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