Production and secretion of heterologous immunomodulators in bacteroides thetaiotaomicron

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dc.contributor.authorTingley, Jeffrey
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSelinger, L. Brent
dc.contributor.supervisorAbbott, D. Wade
dc.date.accessioned2019-09-10T20:46:13Z
dc.date.available2019-09-10T20:46:13Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractChronic and acute inflammation in the colon significantly impact human and animal health, and productivity in agriculture. Unfortunately, current treatments are not very effective for long-term management and treatment is costly. This research project has helped address these limitations by engineering a colonic bacterium, Bacteroides thetaiotaomicron, to produce tumor necrosis factor receptor 2 (TNFR2), α-1-antitrypsin (A1AT) and elafin. These anti-inflammatory proteins were stitched to BtAnchor and BvSecrete signal peptides that facilitate their trafficking to the outer surface and external environment of the cell, respectively. Protein targets were initially produced and localized within the Gram-negative, model organism, Escherichia coli, and elafin demonstrated statistically significant inhibition of elastase. Protein targets: A1AT, TNFR2, and elafin were successfully produced in a dual-glycan responsive B. theta strain previously developed in the Abbott lab. The strains developed as part of this project represent novel tools for reducing of inflammation in agricultural livestock and human medicine.en_US
dc.description.sponsorshipAAFC project ID #1679en_US
dc.identifier.urihttps://hdl.handle.net/10133/5554
dc.language.isoen_USen_US
dc.proquest.subjectMicrobiology [0410]en_US
dc.proquest.subjectAnimal diseases [0476]en_US
dc.proquest.subjectMedicine [0564]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectEscherichia colien_US
dc.subjectInflammatory bowel diseasesen_US
dc.subjectPeptide antibioticsen_US
dc.titleProduction and secretion of heterologous immunomodulators in bacteroides thetaiotaomicronen_US
dc.typeThesisen_US
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