Glycomic analysis of the in vitro modification and utilization of chicken intestinal mucin O-glycan carbohydrates by the enteric poultry pathogen Clostridium perfringens

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dc.contributor.authorMacMillan, Jaclyn L.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbott, D. Wade
dc.contributor.supervisorSelinger, L. Brent
dc.date.accessioned2019-08-28T18:40:06Z
dc.date.available2019-08-28T18:40:06Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractClostridium perfringens is the etiological agent of necrotic enteritis (NE) in poultry. NE occurs within the small intestine and is characterized by intestinal mucus depletion and tissue inflammation. This disease costs the global poultry industry an estimated USD $2-6 billion per year. The small intestine mucus glycans of birds contain a large abundance of sialic acid (Neu5Ac) and sulfate, which may impede colonization by enteric avian bacterial pathogens. C. perfringens was shown here to liberate and metabolize Neu5Ac from extracted broiler chicken mucin O-glycans. By correlating these findings with carbohydrate active enzymes active on mucin O-glycans and the core metabolic enzymes present within C. perfringens, a biochemical basis for these relationships can be established. These new findings suggest that Neu5Ac utilization is significant for mucus colonization by C. perfringens and may provide C. perfringens with a competitive advantage in the avian intestineen_US
dc.embargoNoen_US
dc.embargo.end2019-08-28
dc.embargo.start2018-08-28
dc.identifier.urihttps://hdl.handle.net/10133/5532
dc.language.isoen_USen_US
dc.proquest.subject0306en_US
dc.proquest.subject0487en_US
dc.proquest.subject0410en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectMucinen_US
dc.subjectCarbohydrateen_US
dc.subjectGlycanen_US
dc.subjectIntestinal Mucusen_US
dc.subjectClostridium perfringensen_US
dc.subjectBroiler chickenen_US
dc.subjectPorcine gastric mucinen_US
dc.subjectDissertations, Academicen_US
dc.titleGlycomic analysis of the in vitro modification and utilization of chicken intestinal mucin O-glycan carbohydrates by the enteric poultry pathogen Clostridium perfringensen_US
dc.typeThesisen_US
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