Investigating prokaryotic translation elongation and initiation

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dc.contributor.authorRoberts, Luc Arden Hamilton
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2023-10-12T21:20:54Z
dc.date.available2023-10-12T21:20:54Z
dc.date.issued2023
dc.degree.levelPh.D
dc.description.abstractProtein synthesis or translation is a process critical to life performed by all living organisms. The rapid and accurate production of proteins allows organisms to perform cellular functions, reproduce, and react to environmental stimuli. The core theme of this thesis is prokaryotic translation, beginning with building tools to study the structural dynamics of elongation factor thermo unstable (EF-Tu) and using them to determine the order of events during its functional cycle. Additionally, the phenomenon of prokaryotic structure-based translation initiation by the intergenic region internal ribosome entry site was investigated and its mode of action determined to be different from the previously proposed model. Finally, the preparation and biophysical characterization of the translation initiation region of the rpsA gene from Escherichia coli was performed to begin the work towards an atomic level understanding of this mRNA molecule bound to the prokaryotic ribosome. Together, this thesis demonstrates the robustness of the prokaryotic translation machinery and highlights that, although significant insight has been gained into how these molecular machines operate, there is still additional information needed to fully understand this fundamental process.
dc.identifier.urihttps://hdl.handle.net/10133/6602
dc.language.isoen
dc.proquest.subject0786
dc.proquest.subject0307
dc.proquest.subject0487
dc.proquestyesYes
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectbiochemistry
dc.subjectRNA
dc.subjectprokaryotic translation
dc.subjectEF-Tu
dc.subjecttranslation machinery
dc.subjectprotein synthesis
dc.subjectprotein translation
dc.subjecttranslation initiation
dc.subject.lcshProteins--Synthesis--Research
dc.subject.lcshProkaryotes--Genetics--Research
dc.subject.lcshGenetic translation--Research
dc.subject.lcshRNA--Synthesis
dc.subject.lcshEscherichia coli--Genetics--Research
dc.subject.lcshG proteins--Research
dc.subject.lcshRibosomes
dc.subject.lcshGenetic regulation--Research
dc.subject.lcshGenetic transcription--Regulation--Research
dc.subject.lcshDissertations, Academic
dc.titleInvestigating prokaryotic translation elongation and initiation
dc.typeThesis
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