Non-Boolean characterization of Homer1a intranuclear transcription foci

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dc.contributor.authorWitharana, Wing Kar Li
dc.date.accessioned2014-04-17T01:29:59Z
dc.date.available2014-04-17T01:29:59Z
dc.date.issued2011
dc.degree.levelMasters
dc.descriptionxvi, 125 leaves : ill. ; 29 cmen_US
dc.description.abstractActivity-induced immediate-early gene (IEG) transcription foci can be labelled with fluorescent probes, permitting high temporal and spatial resolution in mapping neuronal circuits. Previous quantification approaches have assumed a Boolean function of transcription foci, assuming that cells are either active or inactive. Due to multiple amplification steps in the in situ hybridization process, it was thought that information relating to magnitudes of firing rates was lost. However, the current data suggest that transcription foci actually exhibit non-Boolean intensity and size values which vary according to behavioural condition. Systematic characterization of transcription foci intensity and size revealed incremental variations such that: home-cage < one-environment exposure < five-environment exposure < maximal electroconvulsive shock. Visual differences in transcription foci may result from a quantifiable relationship between spiking patterns and transcription rates. The exact stoichiometry between neuronal spiking and transcription is not yet clear, but these results suggest that Boolean applications of IEG imaging may neglect accurate neuronal activation properties.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3402
dc.language.isoen_CAen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Neuroscience, c2011en_US
dc.publisher.departmentDepartment of Neuroscienceen_US
dc.publisher.facultyArts and Scienceen_US
dc.subjectScaffold proteins -- Researchen_US
dc.subjectNeuroplasticityen_US
dc.subjectTranscription factorsen_US
dc.subjectNeural circuitryen_US
dc.subjectHippocampus (Brain)en_US
dc.subjectRats as laboratory animalsen_US
dc.subjectFluorescence in situ hybridizationen_US
dc.subjectDissertations, Academicen_US
dc.titleNon-Boolean characterization of Homer1a intranuclear transcription focien_US
dc.typeThesisen_US
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