Investigation of the structure and digestibility of fluorescently labeled carbohydrates using glycoside hydrolases

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dc.contributor.authorAnele, Anuoluwapo
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbott, D. Wade
dc.contributor.supervisorSelinger, L. Brent
dc.date.accessioned2016-11-03T15:28:58Z
dc.date.available2016-11-03T15:28:58Z
dc.date.issued2016
dc.degree.levelMastersen_US
dc.description.abstractDeveloping alternatives to the use of antimicrobials is essential to maintaining the sustainability of animal production and lowering the prevalence of antimicrobial resistance. Prebiotics have not been readily adopted due to limited knowledge of the mechanisms driving prebiotic-bacteria-host interactions. Presented here are preliminary insights into the utility of three prebiotic compounds: neoagarooligosaccharides (NAOS) mannooligosaccharides (MOS), and pectic oligosaccharides (POS). The activity of three agarases from Bacteroides uniformis NP1: BuGH86 (an endolytic enzyme that produces NAOS from agarose), as well as BuGH2C and BuGH117B (exolytic enzymes that remove 3,6-anhydro-L-galactose from the non-reducing end of NAOS and D-galactose from the non-reducing end of agarooligosaccharides, respectively) were determined. NAOS, MOS and POS were then used as substrates to generate fluorescent probes for evaluating interactions with bacterial enzymes. For NAOS and MOS, the digestion of ANTS-labeled oligosaccharides was similar to that of native versions; however, digestion of ANTS-labeled POS was considerably hindered.en_US
dc.embargoYesen_US
dc.embargo.end2016-10-31
dc.embargo.start2016-05-01
dc.identifier.urihttps://hdl.handle.net/10133/4650
dc.language.isoen_CAen_US
dc.proquest.subject0307en_US
dc.proquest.subject0475en_US
dc.proquest.subject0470en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectPrebioticen_US
dc.subjectGlycoside hydrolasesen_US
dc.subjectFluorescent labeled carbohydratesen_US
dc.subjectAgaroseen_US
dc.subjectNeoagarooligosaccharidesen_US
dc.subjectYeast mannanen_US
dc.subjectMannooligosaccharidesen_US
dc.subjectHomogalacturonanen_US
dc.subjectPectic oligosaccharidesen_US
dc.subjectAlternative growth promotersen_US
dc.subjectEnzyme activityen_US
dc.subjectAntimicrobialsen_US
dc.titleInvestigation of the structure and digestibility of fluorescently labeled carbohydrates using glycoside hydrolasesen_US
dc.typeThesisen_US
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